Directional differentiation of chicken embryonic stem cells into osteoblasts, neuron-like cells and adipocytes
Directional differentiation of chicken embryonic stem cells into osteoblasts, neuron-like cells and adipocytes
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鸡胚胎干细胞定向分化为成骨细胞、神经元样细胞和脂肪细胞
DOI：
10.5897/ajb11.481
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发表时间：
2011-08
影响因子：
--
通讯作者：
Chen, Xiangning
中科院分区：
文献类型：
--
作者：
Zhang, Zhentao;Gao, Bo;Shi, Qingqing;Yin, Yanhui;Fu, Dezhi;Li, Bichun;Zhang, Yani;Chen, Guohong;Chen, Xiangning
鸡胚胎干（ES）细胞可用于生产转基因鸡和保存禽类物种中的遗传物质。本研究在体外研究了鸡ES细胞的分化潜力。将鸡ES细胞分别在含地塞米松（DEX）、β-甘油磷酸盐（β-GP）和/或维生素C（Vit C）的诱导培养基中培养15 ~ 21天，分化为成骨细胞，经Von Kossa、细胞化学和免疫组化染色证实，分化率为40 ~ 72%。鸡胚胎干细胞在含有维甲酸（RA）和3-异丁基-1-甲基黄嘌呤（IBMX）的诱导培养基中培养3 ~ 7 d，分化为神经元样细胞，甲苯胺蓝染色和免疫组化染色鉴定分化率为70 ~ 84%。在含有DEX、Ins和/或IBMX的诱导培养基中培养21 d，鸡ES细胞分化为脂肪细胞，油红-O和过氧化物酶体增殖物激活受体-γ（过氧化物酶体增殖物激活受体-γ，PPAR-γ）基因表达的逆转录-聚合酶链反应（RT-PCR）鉴定分化率为74 ~ 91%。这些数据表明，与哺乳动物ES一样，鸡ES可以在体外分化为不同的细胞类型。关键词：鸡胚胎干细胞，体外，定向分化，成骨细胞，神经元样细胞，脂肪细胞。
Chicken embryonic stem (ES) cells are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, the differentiation potential of chicken ES cells was investigated in vitro . Chicken ES cells were differentiated into osteoblasts cultured for 15 to 21 days in the induction media containing dexamethasone (DEX), β-glycerol phosphate (β-GP) and/or vitamin C (Vit C) respectively and differentiation rates ranging from 40 to 72% confirmed by Von Kossa, cytochemical and immunohistochemical staining. Chicken ES differentiated into neuron-like cells cultured for 3 to 7 days in the induction media containing retinoic acid (RA) and 3-isobutyl-1-methylxanthine (IBMX) and differentiation rates ranging from 70 to 84% were identified by toluidine blue staining and immunohistochemical staining. Also chicken ES was differentiated into adipocytes cultured for 21 days in the induction media containing DEX, insulin (Ins) and/or IBMX and differentiation rates ranging from 74 to 91% identified by oil red-O and reverse transcriptase-polymerase chain reaction (RT-PCR) for peroxisome proliferator activated receptor-γ (PPAR-γ) gene expression. These data suggest that like mammalian ES, chicken ES can differentiate into different cell types in vitro . Key words: Chicken embryonic stem cells, in vitro, directional differentiation, osteoblasts, neuron-like cells, adipocytes.
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影响因子：
2.7
作者：
Okada, Y;Shimazaki, T;Okano, H
通讯作者：
Okano, H
DOI：
10.1152/ajpcell.1999.277.1.c132
发表时间：
1999-07-01
影响因子：
5.5
作者：
Otsuka, E;Yamaguchi, A;Hagiwara, H
通讯作者：
Hagiwara, H
影响因子：
4.1
作者：
K. Yagami;Yohei Uyama;Y. Yoshizawa;S. Kakuta;A. Yamaguchi;M. Nagumo
通讯作者：
K. Yagami;Yohei Uyama;Y. Yoshizawa;S. Kakuta;A. Yamaguchi;M. Nagumo
DOI：
--
发表时间：
2007
期刊：
Animal Husbandry & Veterinary Medicine
影响因子：
--
作者：
Liu Bi-chun
通讯作者：
Liu Bi-chun
DOI：
10.1073/pnas.91.12.5330
发表时间：
1994-06-07
影响因子：
11.1
作者：
BANG, YJ;PIRNIA, F;TREPEL, JB
通讯作者：
TREPEL, JB