Intrinsic Cell Polarity Coupled to Growth Axis Formation in Tobacco BY-2 Cells.
Intrinsic Cell Polarity Coupled to Growth Axis Formation in Tobacco BY-2 Cells.
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DOI：
10.1016/j.cub.2020.09.036
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发表时间：
2020-12-21
期刊：
影响因子：
--
通讯作者：
Coen E
中科院分区：
文献类型：
--
作者：
Chan J;Mansfield C;Clouet F;Dorussen D;Coen E
几种植物蛋白优先定位于细胞的一端，允许为细胞分配一种极性。这些细胞极性蛋白通常表现出相邻细胞之间的协调模式，称为组织细胞极性。组织细胞的极性在植物中广泛存在，可以影响细胞的生长、分裂和分化。然而，目前尚不清楚细胞极性是通过细胞内在机制还是外在机制建立的，也不清楚极性是如何与生长相耦合的。为了解决这些问题，我们分析了组织细胞极性蛋白BASL(打破气孔谱系中的不对称)在简化培养细胞细丝和再生前和再生过程中的行为。我们表明，当BASL在烟草BY-2细胞培养物中异位表达时，BASL是两极定位的。异位的BASL优先发现在细胞细丝的发育顶端，可能标志着一个极化的分子地址。极性可以在细胞周期中改变，并且对微管、肌动蛋白或生长素运输抑制剂的治疗具有抵抗力。BASL在球形原生质体中也表现出极性定位，与迄今测试的其他极性蛋白质相反。原生质体中的碱基极性是动态的，对生长素运输抑制剂具有抵抗力。随着原生质体的再生，在各向同性生长的细胞中，极性保持动态，但在各向异性细胞中变得固定，并与细胞生长的轴线一致。我们的发现表明，植物细胞具有固有的极化能力，环境或发育线索可能通过偏向这一极性的方向，从而使各向异性生长的方向发生偏向。异位GFP-BASL揭示了烟草悬浮细胞中一个极化的分子地址，球形原生质体具有固有的极化能力，与再生原生质体的生长各向异性一致，再生原生质体生长各向异性，极性是动态的，各向同性生长。通过GFP-BASL的异位表达揭示烟草悬浮细胞中一个极化的分子地址。它们表明，原生质体具有内在的极化能力，并且在再生时，极性与生长轴对齐。组织范围内的线索可能会偏离极性的方向，从而定向各向异性生长。
Several plant proteins are preferentially localized to one end of a cell, allowing a polarity to be assigned to the cell. These cell polarity proteins often exhibit coordinated patterns between neighboring cells, termed tissue cell polarity. Tissue cell polarity is widespread in plants and can influence how cells grow, divide, and differentiate. However, it is unclear whether cell polarity is established through cell-intrinsic or -extrinsic mechanisms and how polarity is coupled to growth. To address these issues, we analyzed the behavior of a tissue cell polarity protein BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE) in the simplifying context of cultured cell filaments and in protoplasts before and during regeneration. We show that BASL is polarly localized when ectopically expressed in tobacco BY-2 cell cultures. Ectopic BASL is found preferentially at the developing tips of cell filaments, likely marking a polarized molecular address. Polarity can shift during the cell cycle and is resistant to treatment with microtubule, actin or auxin transport inhibitors. BASL also exhibits polar localization in spherical protoplasts, in contrast to other polarity proteins so far tested. BASL polarity within protoplasts is dynamic and resistant to auxin transport inhibitors. As protoplasts regenerate, polarity remains dynamic in isotropically growing cells but becomes fixed in anisotropic cells and aligns with the axis of cell growth. Our findings suggest that plant cells have an intrinsic ability to polarize and that environmental or developmental cues may act by biasing the direction of this polarity and thus the orientation of anisotropic growth. Ectopic GFP-BASL reveals a polarized molecular address in tobacco suspension cells Spherical protoplasts have an intrinsic ability to polarize Polarity is aligned with growth in regenerating protoplasts growing anisotropically Polarity is dynamic in regenerating protoplasts growing isotropically Chan et al. reveal a polarized molecular address in tobacco suspension cells through ectopic expression of GFP-BASL. They show protoplasts have an intrinsic ability to polarize and that polarity is aligned with the growth axis as they regenerate. Tissue-wide cues may bias the direction of polarity and thus orient anisotropic growth.
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影响因子：
3.5
作者：
Dory M;Hatzimasoura E;Kállai BM;Nagy SK;Jäger K;Darula Z;Nádai TV;Mészáros T;López-Juez E;Barnabás B;Palme K;Bögre L;Ditengou FA;Dóczi R
通讯作者：
Dóczi R
影响因子：
6.2
作者：
Biedroń M;Banasiak A
通讯作者：
Banasiak A
影响因子：
7.2
作者：
Aubert, Anne;Marion, Jessica;Satiat-Jeunemaitre, Beatrice
通讯作者：
Satiat-Jeunemaitre, Beatrice
DOI：
10.1073/pnas.0510457103
发表时间：
2006-01-31
影响因子：
11.1
作者：
Smith, RS;Guyomarc'h, S;Prusinkiewicz, P
通讯作者：
Prusinkiewicz, P
BASL控制拟南芥中的不对称细胞分裂。
影响因子：
64.5
作者：
Dong J;MacAlister CA;Bergmann DC
通讯作者：
Bergmann DC