Improving TCSPC data acquisition from CMOS SPAD arrays
Improving TCSPC data acquisition from CMOS SPAD arrays
复制标题
改进 CMOS SPAD 阵列的 TCSPC 数据采集
DOI:
10.1117/12.2032807
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发表时间:
2013
期刊:
影响因子:
--
通讯作者:
Krstajic N
中科院分区:
文献类型:
--
作者:
Krstajic N
We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32× 32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second).
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DOI:
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1999
期刊:
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作者:
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DOI:
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发表时间:
2010
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影响因子:
3.7
作者:
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DOI:
10.1016/s0075-7535(08)00002-8
发表时间:
2009
期刊:
Data Handling in Science and Technology
影响因子:
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作者:
P. Verveer;Q. Hanley
通讯作者:
Q. Hanley
影响因子:
62.1
作者:
Berezin, Mikhail Y.;Achilefu, Samuel
通讯作者:
Achilefu, Samuel