Phase variable expression of capsular polysaccharide modifications allows Campylobacter jejuni to avoid bacteriophage infection in chickens.
Phase variable expression of capsular polysaccharide modifications allows Campylobacter jejuni to avoid bacteriophage infection in chickens.
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DOI：
10.3389/fcimb.2012.00011
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发表时间：
2012
影响因子：
5.7
通讯作者：
Brøndsted L
中科院分区：
文献类型：
--
作者：
Holst Sørensen MC;van Alphen LB;Fodor C;Crowley SM;Christensen BB;Szymanski CM;Brøndsted L
据估计，噬菌体是地球上最丰富的实体，并且可以在其细菌宿主所在的每个生态位中找到。噬菌体与空肠弯曲杆菌（家禽肠道的常见定植菌，也是人类食源性细菌性胃肠炎的主要来源）之间的最初相互作用尚不清楚。最近，我们分离并鉴定了空肠弯曲菌 NCTC11168 的噬菌体 F336 抗性变体，称为 11168R。 11168R 与野生型的比较导致鉴定出一种新型噬菌体受体，即空肠弯曲菌荚膜多糖 (CPS) 的相变 O-氨基磷酸甲酯 (MeOPN) 部分。在这项研究中，我们证明 11168R 菌株已获得与我们收集的其他四种噬菌体（F198、F287、F303 和 F326）的交叉抗性。噬菌斑效率降低表明 MeOPN 被感染空肠弯曲菌的几种噬菌体识别为受体。为了进一步探讨 CPS 修饰在空肠弯曲菌噬菌体识别和感染性中的作用，我们测试了 F198、F287、F303、F326 和 F336 感染 NCTC11168 不同 CPS 变体（包括定义的 CPS 突变体）的能力。这些菌株通过高分辨率魔角旋转核磁共振波谱进行了表征。我们发现，除了 MeOPN 之外，NCTC11168 CPS 结构的相变 3-O-Me 和 6-O-Me 基团也可能影响噬菌体的噬菌斑效率。此外，用空肠弯曲菌 NCTC11168 和噬菌体 F336 共同感染鸡，导致选择出抗性空肠弯曲菌细菌，这些细菌要么缺乏 MeOPN，要么在其表面获得 6-O-Me 基团，这表明可以在体内获得抗性。总之，我们已经证明相变的 CPS 结构调节空肠弯曲菌中的噬菌体感染性，并表明禽类肠道中不断的噬菌体捕食会选择这些结构的变化，从而导致持续的噬菌体-宿主共同进化。
Bacteriophages are estimated to be the most abundant entities on earth and can be found in every niche where their bacterial hosts reside. The initial interaction between phages and Campylobacter jejuni, a common colonizer of poultry intestines and a major source of foodborne bacterial gastroenteritis in humans, is not well understood. Recently, we isolated and characterized a phage F336 resistant variant of C. jejuni NCTC11168 called 11168R. Comparisons of 11168R with the wildtype lead to the identification of a novel phage receptor, the phase variable O-methyl phosphoramidate (MeOPN) moiety of the C. jejuni capsular polysaccharide (CPS). In this study we demonstrate that the 11168R strain has gained cross-resistance to four other phages in our collection (F198, F287, F303, and F326). The reduced plaquing efficiencies suggested that MeOPN is recognized as a receptor by several phages infecting C. jejuni. To further explore the role of CPS modifications in C. jejuni phage recognition and infectivity, we tested the ability of F198, F287, F303, F326, and F336 to infect different CPS variants of NCTC11168, including defined CPS mutants. These strains were characterized by high-resolution magic angle spinning NMR spectroscopy. We found that in addition to MeOPN, the phase variable 3-O-Me and 6-O-Me groups of the NCTC11168 CPS structure may influence the plaquing efficiencies of the phages. Furthermore, co-infection of chickens with both C. jejuni NCTC11168 and phage F336 resulted in selection of resistant C. jejuni bacteria, which either lack MeOPN or gain 6-O-Me groups on their surface, demonstrating that resistance can be acquired in vivo. In summary, we have shown that phase variable CPS structures modulate phage infectivity in C. jejuni and suggest that the constant phage predation in the avian gut selects for changes in these structures leading to a continuing phage–host co-evolution.
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影响因子：
5.7
作者：
Guerry P;Poly F;Riddle M;Maue AC;Chen YH;Monteiro MA
通讯作者：
Monteiro MA
影响因子：
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作者：
HASHEMOLHOSSEINI, S;MONTAG, D;HENNING, U
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通讯作者：
Szymanski, Christine M.
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Monteiro, Mario A.