Campylobacter polysaccharide capsules: virulence and vaccines.
Campylobacter polysaccharide capsules: virulence and vaccines.
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DOI：
10.3389/fcimb.2012.00007
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发表时间：
2012
影响因子：
5.7
通讯作者：
Monteiro MA
中科院分区：
文献类型：
--
作者：
Guerry P;Poly F;Riddle M;Maue AC;Chen YH;Monteiro MA
空肠弯曲杆菌仍然是全球细菌性腹泻的主要原因，并与许多后遗症有关，包括格林巴利综合征、炎症性肠病、反应性关节炎和肠易激综合征。C.空肠对于肠道病原体来说是不寻常的，因为它能够用多糖胶囊（CPS）包覆其表面。这些荚膜多糖在糖组成和键联方面各不相同，特别是涉及不寻常构型的庚糖和O-甲基氨基磷酸酯键联的那些。这种结构多样性与CPS是Penner方案的主要血清决定簇一致，其中有47个C。空肠血清型。CPS表达和修饰的表达都受到滑移链错配修复的相位变化。尽管荚膜是其他病原体的毒力因子，但CPS在C.除了在雪貂疾病模型中证明在血清抗性和腹泻中的作用的描述性研究之外，空肠疾病还没有得到很好的定义。然而，也许在发病机制中的作用的最令人信服的证据是CPS缀合物疫苗在非人灵长类动物中保护免于腹泻疾病的数据。针对这种病原体的CPS结合疫苗方法是有趣的，但需要解决几个问题，包括有效疫苗所需的CPS类型的效价。在发达国家有许多关于CPS血清型流行的研究，但在发病率较高的发展中国家很少有研究。Penner血清分型的复杂性和成本限制了其实用性，最近开发的用于确定胶囊类型的多重PCR方法提供了一种更快速和负担得起的方法的潜力。比较研究表明，这两种方法有很强的相关性，研究开始确定CPS类型在世界范围内的分布，以及检查疾病严重程度与特定CPS类型的相关性。
Campylobacter jejuni remains a major cause of bacterial diarrhea worldwide and is associated with numerous sequelae, including Guillain Barré Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. C. jejuni is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (CPS). These capsular polysaccharides vary in sugar composition and linkage, especially those involving heptoses of unusual configuration and O-methyl phosphoramidate linkages. This structural diversity is consistent with CPS being the major serodeterminant of the Penner scheme, of which there are 47 C. jejuni serotypes. Both CPS expression and expression of modifications are subject to phase variation by slip strand mismatch repair. Although capsules are virulence factors for other pathogens, the role of CPS in C. jejuni disease has not been well defined beyond descriptive studies demonstrating a role in serum resistance and for diarrhea in a ferret model of disease. However, perhaps the most compelling evidence for a role in pathogenesis are data that CPS conjugate vaccines protect against diarrheal disease in non-human primates. A CPS conjugate vaccine approach against this pathogen is intriguing, but several questions need to be addressed, including the valency of CPS types required for an effective vaccine. There have been numerous studies of prevalence of CPS serotypes in the developed world, but few studies from developing countries where the disease incidence is higher. The complexity and cost of Penner serotyping has limited its usefulness, and a recently developed multiplex PCR method for determination of capsule type offers the potential of a more rapid and affordable method. Comparative studies have shown a strong correlation of the two methods and studies are beginning to ascertain CPS-type distribution worldwide, as well as examination of correlation of severity of illness with specific CPS types.
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影响因子：
11.8
作者：
Coker AO;Isokpehi RD;Thomas BN;Amisu KO;Obi CL
通讯作者：
Obi CL
影响因子：
3.2
作者：
Gilbert, Michel;Mandrell, Robert E.;Vinogradov, Evgeny
通讯作者：
Vinogradov, Evgeny
DOI：
10.1111/j.1432-1033.1995.0570d.x
发表时间：
1995-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
ASPINALL, GO;LYNCH, CM;MORAN, AP
通讯作者：
MORAN, AP
影响因子：
29.4
作者：
Gradel, Kim O.;Nielsen, Hans L.;Nielsen, Henrik
通讯作者：
Nielsen, Henrik
影响因子：
5
作者：
de Wit, MAS;Koopmans, MPG;van Duynhoven, YTHF
通讯作者：
van Duynhoven, YTHF