MicroRNAs are differentially abundant during Aedes albopictus diapause maintenance but not diapause induction.
MicroRNAs are differentially abundant during Aedes albopictus diapause maintenance but not diapause induction.
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DOI：
10.1111/imb.12332
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发表时间：
2017-12
影响因子：
2.6
通讯作者：
Armbruster PA
中科院分区：
文献类型：
--
作者：
Batz ZA;Goff AC;Armbruster PA
滞育是一种程序性休眠，使生物体能够通过暂时停止发育和减少新陈代谢来忍受可预测的不利条件。滞育在昆虫中广泛存在，对于生物体在有利的环境条件下协调其生长和繁殖至关重要。虽然滞育的适应意义是很好的理解，滞育的分子机制仍然没有得到解决。我们进行了高通量测序，以研究microRNA在亚洲虎蚊白纹伊蚊滞育中的作用。我们首先研究了miRNAs在滞育诱导中的作用。在滞育诱导和滞育避免条件下的白纹伊蚊。第二，我们通过鉴定滞育和非滞育pharate幼虫中的miRNAs来研究miRNAs在滞育维持中的作用。我们鉴定了162个miRNAs，其中152个是已知的，10个是新发现的。我们在成熟卵母细胞中没有发现差异丰富的miRNAs，在pharate幼虫中发现了7种差异丰富的miRNAs。差异丰度miRNAs的预测目标包括影响与滞育维持相关的几个过程的基因，包括蜕皮激素调节、免疫应答、脂质代谢和发育调节。我们的研究结果表明，Ae.白纹伊蚊在滞育诱导过程中不提供一组独特的miRNAs，但miRNAs是该物种滞育维持的组成部分。
Diapause is a programmed dormancy that allows organisms to tolerate predictable periods of unfavorable conditions by temporarily halting development and reducing metabolism. Diapause is widespread among insects and is crucial for allowing organisms to coordinate their growth and reproduction with favorable environmental conditions. While the adaptive significance of diapause is well-understood, the molecular mechanisms underpinning diapause remain unresolved. We performed high-throughput sequencing to investigate the role of microRNAs in the diapause of the Asian tiger mosquito, Aedes albopictus. We first investigate miRNAs in diapause induction by characterizing maternally-provisioned miRNAs in mature oocytes of Ae. albopictus under diapause-inducing and diapause-averting conditions. Second, we investigate miRNAs in diapause maintenance by characterizing miRNAs in diapause and non-diapause pharate larvae. We identified 162 miRNAs, 152 previously known and 10 putatively novel. We identified no differentially abundant miRNAs in mature oocytes and seven differentially abundant miRNAs in pharate larvae. The predicted targets of differentially abundant miRNAs include genes affecting several processes related to diapause maintenance including ecdysone regulation, immune response, lipid metabolism, and regulation of development. Our results suggest that Ae. albopictus does not maternally-provision a unique set of miRNAs during diapause induction but miRNAs are a component of diapause maintenance in this species.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
9.2
作者：
Boulan, Laura;Martin, David;Milan, Marco
通讯作者：
Milan, Marco
影响因子：
2.1
作者：
HANSON, SM;CRAIG, GB
通讯作者：
CRAIG, GB
影响因子：
2.1
作者：
Benedict, Mark Q.;Levine, Rebecca S.;Lounibos, L. Philip
通讯作者：
Lounibos, L. Philip
影响因子：
2.3
作者：
Choi, Man-Yeon;Jurenka, Russell A.
通讯作者：
Jurenka, Russell A.