Quantitative Proteomic Analysis Reveals Similarities between Huntington's Disease (HD) and Huntington's Disease-Like 2 (HDL2) Human Brains.
Quantitative Proteomic Analysis Reveals Similarities between Huntington's Disease (HD) and Huntington's Disease-Like 2 (HDL2) Human Brains.
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DOI：
10.1021/acs.jproteome.6b00448
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发表时间：
2016-09-02
影响因子：
4.4
通讯作者：
Ross CA
中科院分区：
文献类型：
--
作者：
Ratovitski T;Chaerkady R;Kammers K;Stewart JC;Zavala A;Pletnikova O;Troncoso JC;Rudnicki DD;Margolis RL;Cole RN;Ross CA
The pathogenesis of HD and HDL2, similar progressive neurodegenerative disorders caused by expansion mutations, remains incompletely understood. No systematic quantitative proteomics studies, assessing global changes in HD or HDL2 human brain, were reported. To address this deficit, we used a stable isotope labeling-based approach to quantify the changes in protein abundances in the cortex of 12 HD and 12 control cases and, separately, of 6 HDL2 and 6 control cases. The quality of the tissues was assessed to minimize variability due to post mortem autolysis. We applied a robust median sweep algorithm to quantify protein abundance and performed statistical inference using moderated test statistics. 1211 proteins showed statistically significant fold changes between HD and control tissues; the differences in selected proteins were verified by Western blotting. Differentially abundant proteins were enriched in cellular pathways previously implicated in HD, including Rho-mediated, actin cytoskeleton and integrin signaling, mitochondrial dysfunction, endocytosis, axonal guidance, DNA/RNA processing, and protein transport. The abundance of 717 proteins significantly differed between control and HDL2 brain. Comparative analysis of the disease-associated changes in the HD and HDL2 proteomes revealed that similar pathways were altered, suggesting the commonality of pathogenesis between the two disorders.
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影响因子：
3.3
作者：
Di Domenico, Fabio;Sultana, Rukhsana;Barone, Eugenio;Perluigi, Marzia;Cini, Chiara;Mancuso, Cesare;Cai, Jian;Pierce, William M.;Butterfield, D. Allan
通讯作者：
Butterfield, D. Allan
影响因子：
25
作者：
Langfelder P;Cantle JP;Chatzopoulou D;Wang N;Gao F;Al-Ramahi I;Lu XH;Ramos EM;El-Zein K;Zhao Y;Deverasetty S;Tebbe A;Schaab C;Lavery DJ;Howland D;Kwak S;Botas J;Aaronson JS;Rosinski J;Coppola G;Horvath S;Yang XW
通讯作者：
Yang XW
影响因子：
4.5
作者：
Bañez-Coronel M;Porta S;Kagerbauer B;Mateu-Huertas E;Pantano L;Ferrer I;Guzmán M;Estivill X;Martí E
通讯作者：
Martí E
影响因子：
3.5
作者：
Engelender, S;Sharp, AH;Ross, CA
通讯作者：
Ross, CA
影响因子：
3.5
作者：
Damiano M;Diguet E;Malgorn C;D'Aurelio M;Galvan L;Petit F;Benhaim L;Guillermier M;Houitte D;Dufour N;Hantraye P;Canals JM;Alberch J;Delzescaux T;Déglon N;Beal MF;Brouillet E
通讯作者：
Brouillet E