Expression of iron binding proteins and hemin binding activity in the dental pathogen Actinobacillus actinomycetemcomitans.
Expression of iron binding proteins and hemin binding activity in the dental pathogen Actinobacillus actinomycetemcomitans.
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牙科病原体放线杆菌伴放线杆菌中铁结合蛋白的表达和血红素结合活性。
DOI：
10.1111/j.1574-6968.1998.tb13037.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
Actis,LA
中科院分区：
文献类型：
--
作者：
Graber,KR;Smoot,LM;Actis,LA
伴生放线杆菌表达一种与淋球菌FbpA铁结合蛋白免疫相关的多肽。此外，还通过Northern印迹分析检测了hitBandhitChomologs的表达。该牙周病原体还表达一种与31-kDaHaemophilus流感病毒蛋白同源的多肽，该蛋白与副血链球菌和鼠疫耶尔森菌的FimA和YfeA蛋白具有氨基酸序列同源性。博塔。放线菌伴生蛋白同系物位于周质间隙内，其合成受培养基中铁和氯化血红素浓度的调节。Southern和Western印迹分析以及分子克隆表明，该细菌中存在一个类似于Fur的阻遏子，它可能控制铁对该细菌基因表达的调控。在氯化血红素或刚果红存在下的培养显示了这种微生物结合氯化血红素的能力。经ANA转化后，在含有刚果红和氯化血红素的琼脂平板上分离出产生红色和棕色菌落的大肠杆菌DH5α克隆，进一步证实了这种结合活性。放线菌伴生基因文库。
Actinobacillus actinomycetemcomitanswas found to express a polypeptide immunologically related to theNeisseria gonorrhoeaeFbpA iron binding protein. In addition, the expression ofhitBandhitChomologs was detected by Northern blot analysis. This periodontal pathogen also expresses a polypeptide homologous to the 31-kDaHaemophilus influenzaeprotein, which shows amino acid sequence homology with the FimA and YfeA proteins fromStreptococcus parasanguisandYersinia pestis, respectively. BothA. actinomycetemcomitansprotein homologs were located within the periplasmic space, and their synthesis was regulated by the iron and hemin concentration of the culture medium. Southern and Western blot analysis together with molecular cloning revealed the presence of a Fur-like repressor, which may control the iron regulation of gene expression in this bacterium. Cultivation in the presence of hemin or Congo red revealed the ability of this organism to bind hemin. This binding activity was further confirmed by isolatingEscherichia coliDH5α clones that produced red and brown colonies on agar plates containing Congo red and hemin, respectively, after transformation with anA. actinomycetemcomitansgene library.
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