Listeria monocytogenes exploits normal host cell processes to spread from cell to cell.
Listeria monocytogenes exploits normal host cell processes to spread from cell to cell.
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DOI：
10.1083/jcb.146.6.1333
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发表时间：
1999-09-20
期刊：
影响因子：
--
通讯作者：
Theriot JA
中科院分区：
文献类型：
--
作者：
Robbins JR;Barth AI;Marquis H;de Hostos EL;Nelson WJ;Theriot JA
细菌病原体单核细胞增生李斯特菌在宿主细胞的细胞质中生长，并使用由细菌蛋白ActA介导的基于肌动蛋白的运动形式在细胞间传播。用L.通过视频显微镜观察单核细胞增生和细菌的细胞间传播。含有细菌的膜结合突起形成的概率随着宿主细胞单层年龄和广泛的细胞-细胞接触的建立而降低。在它们延伸进入受体细胞后，细胞间膜结合的突起经历了一段时间的细菌依赖性的间歇运动，然后它们塌陷成空泡并迅速溶解。突起中的肌动蛋白丝与细菌相关的细胞质肌动蛋白彗星尾相比，周转率降低。受体细胞运动性的恢复需要1-2代细菌。这种延迟可以解释由细菌金属蛋白酶，Mpl的ActA的酸依赖性裂解。重要的是，我们已经观察到，在没有细菌的情况下，相邻MDCK细胞表面片段的低水平内吞作用发生，这意味着细菌的细胞间传播可能利用了内源性的副细胞吞噬过程。
The bacterial pathogen, Listeria monocytogenes, grows in the cytoplasm of host cells and spreads intercellularly using a form of actin-based motility mediated by the bacterial protein ActA. Tightly adherent monolayers of MDCK cells that constitutively express GFP-actin were infected with L. monocytogenes, and intercellular spread of bacteria was observed by video microscopy. The probability of formation of membrane-bound protrusions containing bacteria decreased with host cell monolayer age and the establishment of extensive cell-cell contacts. After their extension into a recipient cell, intercellular membrane-bound protrusions underwent a period of bacterium-dependent fitful movement, followed by their collapse into a vacuole and rapid vacuolar lysis. Actin filaments in protrusions exhibited decreased turnover rates compared with bacterially associated cytoplasmic actin comet tails. Recovery of motility in the recipient cell required 1–2 bacterial generations. This delay may be explained by acid-dependent cleavage of ActA by the bacterial metalloprotease, Mpl. Importantly, we have observed that low levels of endocytosis of neighboring MDCK cell surface fragments occurs in the absence of bacteria, implying that intercellular spread of bacteria may exploit an endogenous process of paracytophagy.
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影响因子：
3.6
作者：
KOCKS, C;MARCHAND, JB;COSSART, P
通讯作者：
COSSART, P
影响因子：
64.8
作者：
BIELECKI, J;YOUNGMAN, P;PORTNOY, DA
通讯作者：
PORTNOY, DA
DOI：
10.1083/jcb.109.6.2817
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bacallao R;Antony C;Dotti C;Karsenti E;Stelzer EH;Simons K
通讯作者：
Simons K
肌动蛋白去聚合因子（ADF/Cofilin）提高了细丝流动率：基于肌动蛋白的运动的影响。
DOI：
10.1083/jcb.136.6.1307
发表时间：
1997-03-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carlier MF;Laurent V;Santolini J;Melki R;Didry D;Xia GX;Hong Y;Chua NH;Pantaloni D
通讯作者：
Pantaloni D
影响因子：
7.8
作者：
Adams, CL;Nelson, WJ;Smith, SJ
通讯作者：
Smith, SJ