Hydroxylated near-infrared BODIPY fluorophores as intracellular pH sensors.
Hydroxylated near-infrared BODIPY fluorophores as intracellular pH sensors.
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DOI：
10.1039/c4an01104j
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发表时间：
2014-10-07
期刊：
影响因子：
--
通讯作者：
Henary M
中科院分区：
文献类型：
--
作者：
Salim MM;Owens EA;Gao T;Lee JH;Hyun H;Choi HS;Henary M
在这项研究中，合成并分析了一系列新型、高灵敏度 BF2 螯合四芳基氮杂二吡咯甲烷染料，适合作为开/关光诱导电子转移调制荧光传感器来测定细胞内 pH 值。新指示剂的乙醇溶液在 696-700 nm 范围内具有最大吸收，在 720 nm 范围内具有最大荧光发射。确定了所研究的氮杂-BODIPY指示剂组的摩尔吸光率和荧光量子产率数据。这些指示剂具有约 80 000 M−1 cm−1 的高摩尔吸收系数和量子产率（高达 18%）。指示剂相应的 pKa 值由吸光度和荧光测量确定，范围为 9.1 至 10.8，具体取决于供电子功能的选择性定位。 aza-BODIPY 指示剂出色的光稳定性使其特别适合长时间测量。基于 pH 7.8 和 pH 9.3 的等吸光点对 PC3 细胞中的活组织进行体外细胞染色，结果表明，某些化合物在 800 nm 处的荧光强度随着 pH 值的增加而增加，而在 700 nm 处的荧光强度则降低。因此，新指标适合在各种分析应用中进行开发和适应。
In this study, a series of new, highly sensitive BF2-chelated tetraarylazadipyrromethane dyes are synthesized and analyzed to be suitable as on/off photo-induced electron transfer modulated fluorescent sensors for determination of intracellular pH. The ethanolic solutions of the new indicators feature absorption maxima in the range of 696–700 nm and a fluorescence emission maximum at 720 nm. Molar absorptivity and fluorescence quantum yield data were determined for the studied set of aza-BODIPY indicators. These indicators have high molar absorption coefficients of ~80 000 M−1 cm−1 and quantum yields (up to 18%). Corresponding pKa values of indicators are determined from absorbance and fluorescence measurements and range from 9.1 to 10.8, depending on the selective positioning of electron-donating functionalities. The excellent photostability of the aza-BODIPY indicators makes them particularly suitable for long duration measurements. The in vitro cellular staining of living tissues in PC3 cells based on the isosbestic point at pH 7.8 and pH 9.3 has been employed which shows an increase in fluorescence intensity at 800 nm with increase in pH for certain compounds and fluorescence intensity decreases at 700 nm. Therefore, the new indicators are suitable for exploitation and adaptation in a diverse range of analytical applications.
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影响因子：
46.9
作者：
Ntziachristos, V;Ripoll, J;Weissleder, R
通讯作者：
Weissleder, R
影响因子：
7.4
作者：
ZEN, JM;PATONAY, G
通讯作者：
PATONAY, G
影响因子：
5.2
作者：
Murtagh, Julie;Frimannsson, Daniel O.;O'Shea, Donal F.
通讯作者：
O'Shea, Donal F.
影响因子：
15
作者：
Gorman, A;Killoran, J;O'Shea, DF
通讯作者：
O'Shea, DF
影响因子：
13.1
作者：
Gómez-Hens, A;Aguilar-Caballos, MP
通讯作者：
Aguilar-Caballos, MP