Identification of hsa_circ_0001445 of a novel circRNA-miRNA-mRNA regulatory network as potential biomarker for coronary heart disease.
Identification of hsa_circ_0001445 of a novel circRNA-miRNA-mRNA regulatory network as potential biomarker for coronary heart disease.
复制标题
DOI：
10.3389/fcvm.2023.1104223
复制
发表时间：
2023
影响因子：
3.6
通讯作者：
Pan, ShangLing
中科院分区：
文献类型：
--
作者：
Dinh, PhongSon;Peng, JunHua;Tran, ThanhLoan;Wu, DongFeng;Tran, ChauMyThanh;Dinh, ThiPhuongHoai;Pan, ShangLing
关键词：
探讨冠心病（CHD）患者外周血白细胞hsa_circ_0001445水平及其相关临床因素，并通过生物信息学分析预测其circRNA-miRNA-mRNA调控网络在CHD发病机制中的作用。对94例冠心病患者（年龄65.96 ± 9.78岁）和126例健康对照者（年龄60.75 ± 8.81岁）的外周血白细胞进行了分离。采用qRT-PCR定量检测circRNA的表达水平，并分析其与CHD临床参数的相关性。通过生物信息学算法和GEO数据集，使用Limma软件包评估差异miRNA表达。cyTargetLinker预测miRNA-mRNA调控网络。采用QuarterProfiler对circRNA网络进行功能富集分析，以研究其在CHD发病机制中的作用。CHD患者外周血白细胞hsa_circ_0001445的表达较健康对照组下调。hsa_circ_0001445表达水平与血红蛋白、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平呈正相关。hsa_circ_0001445表达水平与年龄、中性粒细胞水平呈显著负相关。hsa_circ_0001445低表达对冠心病患者和健康对照者的敏感性为67.5%，特异性为76.6%（p < 0.05）。通过生物信息学分析，共识别出405个基因本体术语。基因和基因组术语的京都百科全书主要集中在PI 3 K-Akt信号通路上。hsa_circ_0001445与三种可能调节KEGG过程中涉及的18个基因的miRNA表达相关：hsa-miR-507、hsa-miR-375- 3 p和hsa-miR-942- 5 p。外周血白细胞hsa_circ_0001445水平可作为冠心病诊断的生物标志物。我们对circRNA-miRNA-mRNA网络的研究表明hsa_circ_0001445在CHD发展中的潜在作用。
To evaluate the hsa_circ_0001445 level in peripheral blood leukocytes of patients with coronary heart disease (CHD) and its related clinical factors, and predict its circRNA-miRNA-mRNA regulatory network in CHD pathogenesis via bioinformatics analysis. Peripheral blood leukocytes were isolated from the whole blood samples of 94 CHD patients (aged 65.96 ± 9.78 years old) and 126 healthy controls (aged 60.75 ± 8.81 years old). qRT-PCR was used to quantify the expression level of circRNA and subsequently analyze its association with CHD clinical parameters. Via bioinformatics algorithm and GEO datasets, differential miRNA expression was evaluated using the Limma package. A miRNA-mRNA regulatory network was predicted by cyTargetLinker. ClusterProfiler was employed to perform functional enrichment analysis of the circRNA network to investigate its role in CHD pathogenesis. The expression of hsa_circ_0001445 in peripheral blood leukocytes of CHD patients was downregulated compared with that of healthy controls. Positive correlations were evident between hsa_circ_0001445 expression level and the levels of hemoglobin, triglycerides, high- and low-density lipoprotein cholesterol. A significant negative correlation was also found between hsa_circ_0001445 expression level and age and the neutrophil level. Low expression of hsa_circ_0001445 exhibited a discriminatory ability between CHD patients and healthy controls with a sensitivity of 67.5% and a specificity of 76.6% (p < 0.05). By bioinformatics analysis, 405 gene ontology terms were identified. The Kyoto Encyclopedia of Genes and Genomes terms focused principally on the PI3K-Akt signaling pathway. hsa_circ_0001445 was associated with the expression of three miRNAs that may regulate 18 genes involved in KEGG processes: hsa-miR-507, hsa-miR-375–3p, and hsa-miR-942–5p. The hsa_circ_0001445 level in peripheral blood leukocytes may serve as a biomarker for CHD diagnosis. Our work on circRNA-miRNA-mRNA networks suggests a potential role for hsa_circ_0001445 in CHD development.
登录
查看更多内容
影响因子：
5.6
作者：
Ghigo A;Li M
通讯作者：
Li M
DOI：
10.1002/stem.2594
发表时间：
2017-04
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Cochrane A;Kelaini S;Tsifaki M;Bojdo J;Vilà-González M;Drehmer D;Caines R;Magee C;Eleftheriadou M;Hu Y;Grieve D;Stitt AW;Zeng L;Xu Q;Margariti A
通讯作者：
Margariti A
影响因子：
4.6
作者：
Barrett, Steven P.;Salzman, Julia
通讯作者：
Salzman, Julia
影响因子：
--
作者：
Liang, Bin;Li, Menglan;Zheng, Fang
通讯作者：
Zheng, Fang
影响因子：
5.5
作者：
Chen X;Yin J;Cao D;Xiao D;Zhou Z;Liu Y;Shou W
通讯作者：
Shou W