Prediction of Selected Biosynthetic Pathways for the Lipopolysaccharide Components in Porphyromonas gingivalis.
Prediction of Selected Biosynthetic Pathways for the Lipopolysaccharide Components in Porphyromonas gingivalis.
复制标题
牙龈卟啉单胞菌脂多糖成分的选定生物合成途径的预测。
DOI：
10.3390/pathogens10030374
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发表时间：
2021-03-20
期刊：
影响因子：
--
通讯作者：
Caspi R
中科院分区：
文献类型：
--
作者：
Swietnicki W;Caspi R
牙龈卟啉单胞菌是一种口腔致病菌。这种细菌会破坏牙齿组织，是一个严重的全球性健康问题。实验数据和生物信息学分析表明，病原体产生三种类型的脂多糖（LPS）：正常（O型），阴离子（A型）和荚膜（K型）。参与生产所有三种类型的脂多糖的酶已经在很大程度上确定了前两种，部分确定了第三种类型。在目前的工作中，我们使用生物信息学工具来预测生物合成途径的正常（O型）脂多糖的生产在W50株牙龈卟啉单胞菌和比较的途径与其他假定的途径在完全测序和完整的基因组的其他致病菌株。从该途径中选择的酶已被建模，并提出了推定的结构。由于文献中提出的两种相互排斥的结构，此时无法预测A型抗原的途径。由于缺乏抗原的结构数据，也无法预测K型抗原生物合成的途径。然而，脂质A的合成途径，其核心成分，和O-型抗原连接酶反应已提出的基础上结合的实验数据和生物信息学分析。预测的途径与已知的途径在其他系统中进行了比较和讨论。这是文献中的第一份报告，详细显示了牙龈卟啉单胞菌模型W50菌株合成选定LPS组分的预测途径。
Porphyromonas gingivalis is an oral human pathogen. The bacterium destroys dental tissue and is a serious health problem worldwide. Experimental data and bioinformatic analysis revealed that the pathogen produces three types of lipopolysaccharides (LPS): normal (O-type), anionic (A-type), and capsular (K-type). The enzymes involved in the production of all three types of lipopolysaccharide have been largely identified for the first two and partially for the third type. In the current work, we use bioinformatics tools to predict biosynthetic pathways for the production of the normal (O-type) lipopolysaccharide in the W50 strain Porphyromonas gingivalis and compare the pathway with other putative pathways in fully sequenced and completed genomes of other pathogenic strains. Selected enzymes from the pathway have been modeled and putative structures are presented. The pathway for the A-type antigen could not be predicted at this time due to two mutually exclusive structures proposed in the literature. The pathway for K-type antigen biosynthesis could not be predicted either due to the lack of structural data for the antigen. However, pathways for the synthesis of lipid A, its core components, and the O-type antigen ligase reaction have been proposed based on a combination of experimental data and bioinformatic analyses. The predicted pathways are compared with known pathways in other systems and discussed. It is the first report in the literature showing, in detail, predicted pathways for the synthesis of selected LPS components for the model W50 strain of P. gingivalis.
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影响因子：
3.4
作者：
Coats SR;Jones JW;Do CT;Braham PH;Bainbridge BW;To TT;Goodlett DR;Ernst RK;Darveau RP
通讯作者：
Darveau RP
将细菌的Vulgatus LPS结构与其对人类细胞模型的免疫调节作用。
影响因子：
18.2
作者：
Di Lorenzo F;Pither MD;Martufi M;Scarinci I;Guzmán-Caldentey J;Łakomiec E;Jachymek W;Bruijns SCM;Santamaría SM;Frick JS;van Kooyk Y;Chiodo F;Silipo A;Bernardini ML;Molinaro A
通讯作者：
Molinaro A
影响因子：
3.1
作者：
Al-Qutub, Montaser N.;Braham, Pamela H.;Darveau, Richard P.
通讯作者：
Darveau, Richard P.
影响因子：
3.5
作者：
OGAWA, T
通讯作者：
OGAWA, T
影响因子：
15
作者：
Morgan, PM;Sala, RF;Tanner, ME
通讯作者：
Tanner, ME