Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models.
Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models.
复制标题
将细菌的Vulgatus LPS结构与其对人类细胞模型的免疫调节作用。
DOI：
10.1021/acscentsci.0c00791
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发表时间：
2020-09-23
影响因子：
18.2
通讯作者：
Molinaro A
中科院分区：
文献类型：
--
作者：
Di Lorenzo F;Pither MD;Martufi M;Scarinci I;Guzmán-Caldentey J;Łakomiec E;Jachymek W;Bruijns SCM;Santamaría SM;Frick JS;van Kooyk Y;Chiodo F;Silipo A;Bernardini ML;Molinaro A
肠道微生物群通过设定免疫激活的全身阈值来指导宿主免疫系统的发育。来自肠道细菌的脂多糖（LPS）能够触发全身和局部促炎和免疫调节反应，并且这种能力强烈依赖于它们的精细结构。到目前为止，只有少数的LPS结构从肠道黏膜已被阐明，因此，可能是重要的LPS-哺乳动物细胞在肠道水平的相互作用的分子基序仍然是模糊的。在这里，我们报告的完整结构的LPS分离出的一个突出的物种的属拟杆菌，普通拟杆菌。LPS原来是由一个特定的化学结构的基础上hypoacylated和单磷酸化的脂质A，并与半乳糖呋喃糖的核心寡糖和O-抗原建立的甘露糖和鼠李糖。对该LPS在人体外模型上的免疫学性质的评价揭示了产生抗炎细胞因子和诱导MD-2/TLR 4-和TLR 2-介导的信号传导途径的协同作用的非常有趣的能力。肠道普通拟杆菌的脂多糖具有新颖的结构特征和独特的免疫学行为，包括温和的激动作用和TLR 2/TLR 4的联合激活。
The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS–mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways. Lipopolysaccharide of the gut commensal Bacteroides vulgatus shows novel structural features and a peculiar immunological behavior including a mild agonistic effect and combined TLR2/TLR4 activation.
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影响因子：
4
作者：
Chiodo, Fabrizio;Marradi, Marco;Tefsen, Boris
通讯作者：
Tefsen, Boris
影响因子：
--
作者：
Chandler, Courtney E;Ernst, Robert K
通讯作者：
Ernst, Robert K
影响因子：
4.4
作者：
Hornung, V;Rothenfusser, S;Hartmann, G
通讯作者：
Hartmann, G
DOI：
10.1111/j.1432-1033.1993.tb18409.x
发表时间：
1993-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BRANDENBURG, K;MAYER, H;SEYDEL, U
通讯作者：
SEYDEL, U
影响因子：
2.9
作者：
KESSLER, RJ;FANESTIL, DD
通讯作者：
FANESTIL, DD