Increased plasma cell-free DNA level during HTNV infection: correlation with disease severity and virus load.
Increased plasma cell-free DNA level during HTNV infection: correlation with disease severity and virus load.
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DOI：
10.3390/v6072723
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发表时间：
2014-07-15
期刊：
影响因子：
--
通讯作者：
Jin B
中科院分区：
文献类型：
--
作者：
Yi J;Zhang Y;Zhang Y;Ma Y;Zhang C;Li Q;Liu B;Liu Z;Liu J;Zhang X;Zhuang R;Jin B
血液中的无细胞DNA（Cell-free DNA，cf-DNA）是由病毒感染或创伤、肿瘤等引发的DNA损伤反应。汉坦病毒主要引起两种疾病：肾综合征出血热（Hemorrhagic Fever with Renal Syndrome，HFRS）和汉坦病毒心肺综合征（Hantavirus Cardiopulmonary Syndrome，HCPS）。本研究旨在探讨HFRS患者急性期血浆cf-DNA水平及其与病情严重程度和血浆汉坦病毒（HTNV）载量的关系。本文观察了76例HFRS患者166份血浆中cf-DNA的出现情况：HFRS患者血浆cf-DNA水平在病程后期达到高峰，以后逐渐下降。至利尿期，患者血浆cf-DNA水平与健康对照组无显著性差异。除发热/水肿期外，重症/危重症患者血浆cf-DNA水平高于轻/中度组。HFRS早期血浆cf-DNA值与HTNV载量及病情严重程度相关。在大多数患者中，血浆cf-DNA显示低分子量外观，对应于凋亡DNA的大小。结论：HFRS患者血浆cf-DNA水平呈动态升高，且与病情严重程度相关，提示血浆cf-DNA可能是HFRS发病和预后的潜在生物标志物。
Cell-free DNA (cf-DNA) in blood represents a promising DNA damage response triggered by virus infection or trauma, tumor, etc. Hantavirus primarily causes two diseases: haemorrhagic fever with renal syndrome (HFRS) and Hantavirus cardiopulmonary syndrome (HCPS), depending on different Hantavirus species. The aim of this study was to evaluate plasma cf-DNA levels in acute phase of HFRS, and to correlate plasma cf-DNA with disease severity and plasma Hanttan virus (HTNV) load. We observed the appearance of cf-DNA in 166 plasma samples from 76 HFRS patients: the plasma cf-DNA levels peaked at the hypotensive stage of HFRS, and then decreased gradually. Until the diuretic stage, there was no significant difference in plasma cf-DNA level between patients and the healthy control. Exclusively in the febrile/hypotensive stage, the plasma cf-DNA levels of severe/critical patients were higher than those of the mild/moderate group. Moreover, the plasma cf-DNA value in the early stage of HFRS was correlated with HTNV load and disease severity. In most of the patients, plasma cf-DNA displayed a low-molecular weight appearance, corresponding to the size of apoptotic DNA. In conclusion, the plasma cf-DNA levels were dynamically elevated during HFRS, and correlated with disease severity, which suggests that plasma cf-DNA may be a potential biomarker for the pathogenesis and prognosis of HFRS.
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影响因子：
5
作者：
Rainer, Timothy H.;Lam, Nicole Y. L.;Lo, Y. M. Dennis
通讯作者：
Lo, Y. M. Dennis
DOI：
10.1196/annals.1368.037
发表时间：
2006-01-01
期刊：
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV
影响因子：
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作者：
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通讯作者：
Tsigas, D.
影响因子：
30.5
作者：
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通讯作者：
Jinushi, Masahisa
影响因子：
4.7
作者：
Chen, Z;Fadiel, A;Xia, Y
通讯作者：
Xia, Y
影响因子：
12.4
作者：
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