Loss of Pluripotency in Human Embryonic Stem Cells Directly Correlates with an Increase in Nuclear Zinc
Loss of Pluripotency in Human Embryonic Stem Cells Directly Correlates with an Increase in Nuclear Zinc
复制标题
人类胚胎干细胞多能性的丧失与核锌的增加直接相关
作者：
J. Wolford;Y. Chishti;Q. Jin;J. Ward;Liaohai Chen;S. Vogt;L. Finney
人类胚胎干细胞 (hESC) 的多能性对于早期发育研究和细胞替代疗法非常重要，但多能性背后的机制尚不完全清楚。锌已被证明在非多能细胞类型的分化中发挥关键作用，但这里直接研究了它在 hESC 中的作用。通过使用 X 射线荧光微探针 (XFM) 高分辨率绘制 hESC 中金属的分布图并分析亚细胞金属含量，我们发现多能性丧失与核锌增加直接相关的证据。锌的升高不仅重新定义了我们对支持多能性机制的理解，而且还可以作为干细胞分化的生物标志物和干预点。
The pluripotency of human embryonic stem cells (hESCs) is important to investigations of early development and to cell replacement therapy, but the mechanism behind pluripotency is incompletely understood. Zinc has been shown to play a key role in differentiation of non-pluripotent cell types, but here its role in hESCs is directly examined. By mapping the distribution of metals in hESCs at high resolution by x-ray fluorescence microprobe (XFM) and by analyzing subcellular metal content, we have found evidence that loss of pluripotency is directly correlated with an increase in nuclear zinc. Zinc elevation not only redefines our understanding of the mechanisms that support pluripotency, but also may act as a biomarker and an intervention point for stem cell differentiation.
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影响因子：
3
作者：
Glesne, David;Vogt, Stefan;Huberman, Eliezer
通讯作者：
Huberman, Eliezer
影响因子：
4
作者：
Gee, KR;Zhou, ZL;Weiss, JH
通讯作者：
Weiss, JH
影响因子：
15
作者：
Burdette, SC;Walkup, GK;Lippard, SJ
通讯作者：
Lippard, SJ
DOI：
10.1073/pnas.0406547102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Yang, LC;McRae, R;Fahrni, CJ
通讯作者：
Fahrni, CJ