High-Throughput Screening of Thiol-ene Click Chemistries for Bone Adhesive Polymers.
High-Throughput Screening of Thiol-ene Click Chemistries for Bone Adhesive Polymers.
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对骨粘合剂聚合物的硫醇 - 二烯的高通量筛选。
DOI：
10.1021/acsami.3c12072
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发表时间：
2023-10-31
影响因子：
9.5
通讯作者：
Celiz, Adam D.
中科院分区：
文献类型：
--
作者：
Ganabady, Kavya;Negrini, Nicola Contessi;Scherba, Jacob C.;Nitschke, Brandon M.;Alexander, Morgan R.;Vining, Kyle H.;Grunlan, Melissa A.;Mooney, David J.;Celiz, Adam D.
全球每年有数以百万计的整形外科手术使用金属外科钉和螺丝，但在复杂的粉碎性骨折或较小骨骼的手术中，它们的可用性有限。因此，长期以来，用骨粘连材料取代这种植入物一直被认为是一个有吸引力的选择。然而，合成一种具有高粘接强度且易于应用的生物相容性骨粘合剂面临着许多挑战。为了快速确定生物相容性骨粘附剂的候选聚合物，我们采用高通量筛选策略来评估人间充质基质细胞(HMSC)与通过硫醇-烯点击化学合成的聚合物库的粘附性。我们选择了硫醇-烯点击化学，因为多官能团单体可以通过紫外光快速固化，同时最大限度地减少残留单体，而且它为从高通量屏幕识别的候选聚合物提供了一种可扩展的制造工艺。这种筛选方法确定了一种由单体1，3，5-triallyl-1，3，5-triazine-2，4，6(1H，3H，5H)-trione(TATATO)和季戊四醇四(3-巯基丙酸酯)(PETMP)组成的共聚物(1-S2-FT01)，它支持hMSC在90种聚合物库中的最高粘附性。与现有的商用骨粘合剂相比，已鉴定的共聚物(1-S2-FT01)表现出良好的压缩和拉伸性能，并以类似于商业骨胶(如组丙烯)的粘合强度粘合在骨上。此外，这种细胞相容的聚合物支持hMSCs的成骨分化，并可以将3D多孔聚合物支架附着在骨组织上，使其成为一种理想的替代骨粘合剂，在骨科手术中具有广泛的用途。
Metal surgical pins and screws are employed in millions of orthopedic surgical procedures every year worldwide, but their usability is limited in the case of complex, comminuted fractures or in surgeries on smaller bones. Therefore, replacing such implants with a bone adhesive material has long been considered an attractive option. However, synthesizing a biocompatible bone adhesive with a high bond strength that is simple to apply presents many challenges. To rapidly identify candidate polymers for a biocompatible bone adhesive, we employed a high-throughput screening strategy to assess human mesenchymal stromal cell (hMSC) adhesion toward a library of polymers synthesized via thiol–ene click chemistry. We chose thiol–ene click chemistry because multifunctional monomers can be rapidly cured via ultraviolet (UV) light while minimizing residual monomer, and it provides a scalable manufacturing process for candidate polymers identified from a high-throughput screen. This screening methodology identified a copolymer (1-S2-FT01) composed of the monomers 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione (TATATO) and pentaerythritol tetrakis (3-mercaptopropionate) (PETMP), which supported highest hMSC adhesion across a library of 90 polymers. The identified copolymer (1-S2-FT01) exhibited favorable compressive and tensile properties compared to existing commercial bone adhesives and adhered to bone with adhesion strengths similar to commercially available bone glues such as Histoacryl. Furthermore, this cytocompatible polymer supported osteogenic differentiation of hMSCs and could adhere 3D porous polymer scaffolds to the bone tissue, making this polymer an ideal candidate as an alternative bone adhesive with broad utility in orthopedic surgery.
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DOI：
10.1002/adma.201501351
发表时间：
2015-07-15
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Celiz AD;Smith JG;Patel AK;Hook AL;Rajamohan D;George VT;Flatt L;Patel MJ;Epa VC;Singh T;Langer R;Anderson DG;Allen ND;Hay DC;Winkler DA;Barrett DA;Davies MC;Young LE;Denning C;Alexander MR
通讯作者：
Alexander MR
DOI：
10.1039/d0tb02987d
发表时间：
2021-05-14
期刊：
Journal of materials chemistry. B
影响因子：
--
作者：
Pfau MR;McKinzey KG;Roth AA;Graul LM;Maitland DJ;Grunlan MA
通讯作者：
Grunlan MA
影响因子：
5
作者：
White K;Chalaby R;Lowe G;Berlin J;Glackin C;Olabisi R
通讯作者：
Olabisi R
影响因子：
1.8
作者：
Vieira, Juliana de Souza;Santos, Felipe Rychuv;de Araujo, Melissa Rodrigues
通讯作者：
de Araujo, Melissa Rodrigues
影响因子：
13.6
作者：
Sani, Ehsan Shirzaei;Kheirkhah, Ahmad;Annabi, Nasim
通讯作者：
Annabi, Nasim