Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside.
Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside.
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DOI：
10.1126/sciadv.abq3817
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发表时间：
2022-09-09
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
--
作者：
微管通过在体内形成双联体和分支结构等上层结构，在生物功能中发挥重要作用。尽管很重要，但在体外构建这些上层结构仍具有挑战性。在这里，我们设计了一种与 His 标签和 Tau 衍生肽 (TP) 融合的四聚体荧光蛋白 Azami-Green (AG)，TP-AG，以生成超结构。通过改变聚合条件，可以将TP-AG的主要结合位点控制在微管的内部和外部。 TP-AG与内部的结合促进了微管的形成并产生刚性且稳定的微管。 TP-AG与外部的结合通过将微管蛋白招募到微管中来诱导各种微管超结构，包括双联体、多重联体、分支结构和极长的微管。 TP-AG 还构建了活动微管 aster 结构。由单一类型的外源蛋白产生多种微管超结构是理解微管功能和构建基于微管的纳米材料的新概念。肽融合四聚体蛋白诱导稳定微管超结构的产生，例如双联体和分支。
Microtubules play important roles in biological functions by forming superstructures, such as doublets and branched structures, in vivo. Despite the importance, it is challenging to construct these superstructures in vitro. Here, we designed a tetrameric fluorescent protein Azami-Green (AG) fused with His-tag and Tau-derived peptide (TP), TP-AG, to generate the superstructures. Main binding sites of TP-AG can be controlled to the inside and outside of microtubules by changing the polymerization conditions. The binding of TP-AG to the inside promoted microtubule formation and generated rigid and stable microtubules. The binding of TP-AG to the outside induced various microtubule superstructures, including doublets, multiplets, branched structures, and extremely long microtubules by recruiting tubulins to microtubules. Motile microtubule aster structures were also constructed by TP-AG. The generation of various microtubule superstructures by a single type of exogenous protein is a new concept for understanding the functions of microtubules and constructing microtubule-based nanomaterials. Peptide-fused tetrameric proteins induce the generation of stable microtubule superstructures such as doublets and branches.
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影响因子：
16.6
作者：
Keya JJ;Suzuki R;Kabir AMR;Inoue D;Asanuma H;Sada K;Hess H;Kuzuya A;Kakugo A
通讯作者：
Kakugo A
影响因子：
21.3
作者：
Basnet N;Nedozralova H;Crevenna AH;Bodakuntla S;Schlichthaerle T;Taschner M;Cardone G;Janke C;Jungmann R;Magiera MM;Biertümpfel C;Mizuno N
通讯作者：
Mizuno N
影响因子：
64.5
作者：
Case, RB;Pierce, DW;Vale, RD
通讯作者：
Vale, RD
DOI：
10.1016/j.bbrc.2008.04.135
发表时间：
2008-07-11
影响因子：
3.1
作者：
Arii, Yusuke;Hatori, Kuniyuki
通讯作者：
Hatori, Kuniyuki
影响因子：
3.3
作者：
Chaaban S;Brouhard GJ
通讯作者：
Brouhard GJ