Brassinosteroids regulate pavement cell growth by mediating BIN2-induced microtubule stabilization.
Brassinosteroids regulate pavement cell growth by mediating BIN2-induced microtubule stabilization.
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油菜素类固醇通过介导 BIN2 诱导的微管稳定来调节路面细胞生长
DOI：
10.1093/jxb/erx467
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发表时间：
2018-02-23
影响因子：
6.9
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Liu X;Yang Q;Wang Y;Wang L;Fu Y;Wang X
通过与油菜素甾醇不敏感2（BIN 2）（油菜素甾醇反应的负调节剂）相互作用来稳定微管，将油菜素甾醇信号传导与细胞骨架动力学和植物发育的调节联系起来。植物甾醇类化合物（BRs）是一类植物甾体激素，在植物生长发育中起着重要的调控作用。细胞骨架也影响关键的发育过程，BR生物合成或信号传导的缺陷导致与微管缺陷突变体相似的异常表型。然而，BRs如何调节微管和细胞形态仍不清楚。在这里，使用液相色谱-串联质谱法，我们确定了微管蛋白与拟南芥油菜素不敏感2（BIN 2），在植物中BR反应的负调节相互作用。体外和体内下拉测定证实了BIN 2与微管蛋白相互作用。高速共沉淀试验表明，BIN 2也结合微管。拟南芥基因组还编码两个BIN 2同源物，BIN 2-LIKE 1（BIL 1）和BIL 2，其与BIN 2冗余地起作用。在bin 2 -3 bil 1 bil 2三重突变体，皮质微管更敏感的微管破坏药物安磺灵比野生型治疗，而在BIN 2功能获得性突变体bin 2 -1，皮质微管不敏感安磺灵治疗。这些结果提供了重要的洞察如何BR调节植物铺面细胞和叶片生长介导的稳定微管的BIN 2。
The stabilization of microtubules by interaction with BRASSINOSTEROID INSENSITIVE2 (BIN2), a negative regulator of brassinosteroid responses, links brassinosteroid signaling to cytoskeletal dynamics and the regulation of plant development. Brassinosteroids (BRs), a group of plant steroid hormones, play important roles in regulating plant development. The cytoskeleton also affects key developmental processes and a deficiency in BR biosynthesis or signaling leads to abnormal phenotypes similar to those of microtubule-defective mutants. However, how BRs regulate microtubule and cell morphology remains unknown. Here, using liquid chromatography–tandem mass spectrometry, we identified tubulin proteins that interact with Arabidopsis BRASSINOSTEROID INSENSITIVE2 (BIN2), a negative regulator of BR responses in plants. In vitro and in vivo pull-down assays confirmed that BIN2 interacts with tubulin proteins. High-speed co-sedimentation assays demonstrated that BIN2 also binds microtubules. The Arabidopsis genome also encodes two BIN2 homologs, BIN2-LIKE 1 (BIL1) and BIL2, which function redundantly with BIN2. In the bin2-3 bil1 bil2 triple mutant, cortical microtubules were more sensitive to treatment with the microtubule-disrupting drug oryzalin than in wild-type, whereas in the BIN2 gain-of-function mutant bin2-1, cortical microtubules were insensitive to oryzalin treatment. These results provide important insight into how BR regulates plant pavement cell and leaf growth by mediating the stabilization of microtubules by BIN2.
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影响因子：
16.6
作者：
Gomez JM;Chumakova L;Bulgakova NA;Brown NH
通讯作者：
Brown NH
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作者：
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通讯作者：
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