Systematic Analysis of the 4-Coumarate:Coenzyme A Ligase (4CL) Related Genes and Expression Profiling during Fruit Development in the Chinese Pear.
Systematic Analysis of the 4-Coumarate:Coenzyme A Ligase (4CL) Related Genes and Expression Profiling during Fruit Development in the Chinese Pear.
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作者：
Cao Y;Han Y;Li D;Lin Y;Cai Y
在植物中，4-香豆酸：辅酶A连接酶（4CL），包括一些腺苷酸形成酶，是参与调节木质素代谢以及类黄酮和其它次生代谢物的生物合成的关键酶。虽然一些4CL相关蛋白被证明在次生代谢中发挥作用，但在梨和其他蔷薇科物种中还没有全面的4CL相关基因的研究报道。在这项研究中，我们确定了苹果，桃，杨梅，梨基因组中的4CL相关基因使用DNATOOLS软件，并推断其进化关系，系统发育分析，共线性分析，保守基序分析，结构分析。在蔷薇科4种植物（梨、苹果、桃和杨梅）中共鉴定出149个4CL相关基因，其中梨有30个。采用实时荧光定量PCR（qRT-PCR）技术研究了4-CL和酰基辅酶A合成酶（ACS）基因在梨果实发育过程中的功能。我们发现，在梨的30个4CL相关基因发生了重复事件。这些重复的4CL相关基因在梨的所有染色体上分布不均匀，除了第4、8、11和12号染色体。本研究结果为进一步研究4CL相关基因的功能和进化历史奠定了基础。
In plants, 4-coumarate:coenzyme A ligases (4CLs), comprising some of the adenylate-forming enzymes, are key enzymes involved in regulating lignin metabolism and the biosynthesis of flavonoids and other secondary metabolites. Although several 4CL-related proteins were shown to play roles in secondary metabolism, no comprehensive study on 4CL-related genes in the pear and other Rosaceae species has been reported. In this study, we identified 4CL-related genes in the apple, peach, yangmei, and pear genomes using DNATOOLS software and inferred their evolutionary relationships using phylogenetic analysis, collinearity analysis, conserved motif analysis, and structure analysis. A total of 149 4CL-related genes in four Rosaceous species (pear, apple, peach, and yangmei) were identified, with 30 members in the pear. We explored the functions of several 4CL and acyl-coenzyme A synthetase (ACS) genes during the development of pear fruit by quantitative real-time PCR (qRT-PCR). We found that duplication events had occurred in the 30 4CL-related genes in the pear. These duplicated 4CL-related genes are distributed unevenly across all pear chromosomes except chromosomes 4, 8, 11, and 12. The results of this study provide a basis for further investigation of both the functions and evolutionary history of 4CL-related genes.
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DOI：
10.1073/pnas.0900906106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Fawcett, Jeffrey A.;Maere, Steven;Van de Peer, Yves
通讯作者：
Van de Peer, Yves
影响因子：
3.7
作者：
Cukovic, D;Ehlting, J;Douglas, CJ
通讯作者：
Douglas, CJ
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
5.6
作者：
Cao Y;Han Y;Li D;Lin Y;Cai Y
通讯作者：
Cai Y
影响因子：
7.4
作者：
Allina, SM;Pri-Hadash, A;Douglas, CJ
通讯作者：
Douglas, CJ