Arsenic methylation by a genetically engineered Rhizobium-legume symbiont.
Arsenic methylation by a genetically engineered Rhizobium-legume symbiont.
复制标题
基因工程根瘤菌-豆科植物共生体的砷甲基化。
DOI：
10.1007/s11104-017-3207-z
复制
发表时间：
2017-07
期刊：
影响因子：
4.9
通讯作者：
Zhao FJ
中科院分区：
文献类型：
--
作者：
Zhang J;Xu Y;Cao T;Chen J;Rosen BP;Zhao FJ
砷（As）是最广泛的环境污染物之一。本研究的目的是测试一种基于豆科植物与基因工程根瘤菌共生的新型生物修复系统。将来源于莱茵衣藻（Chlamydiumreinhardtii）的砷[As（III）] S-腺苷甲硫氨酸甲基转移酶基因（CrarsM）插入到豆科根瘤菌（Rhizobiumleguminosarumbv.三叶草菌株R3。在自由生活条件下和与红三叶草植物共生的情况下测试重组根瘤菌的As甲基化能力。砷的形态测定采用高效液相色谱-电感耦合等离子体质谱法。在自由生活条件下，CrarsM重组R.豆科植物获得了将As（III）甲基化为甲基化砷化合物的能力，包括甲基胂酸盐[MAs（V）]、二甲基胂酸盐[DMAs（V）]和氧化三甲基砷[TMAs（V）O]。将红三叶草植物用对照（非重组）或CrarsM重组R.豆胶菌（leguminosarum），暴露于5或10 μM亚砷酸盐中。在接种对照R的红三叶草植株中未检测到甲基化的As。豆科植物与此相反，重组根瘤菌与红三叶草共生后，在根瘤和地上部中均检测到3种甲基化产物，分别占两种植物组织中总砷的74.7-75.1%和29.1-42.4%。重组共生体也挥发出少量的As。本研究表明，工程根瘤菌表达的藻类arsM基因可以甲基化和挥发砷，提供了一个潜在的未来使用的豆类根瘤菌共生体作为生物修复的概念证明。
Arsenic (As) is one of the most widespread environmental contaminants. The aim of our study was to test a novel bioremediation system based on the symbiosis between leguminous plant and genetically engineered rhizobia. The arsenite [As(III)] S-adenosylmethionine methyltransferase gene (CrarsM) from the alga Chlamydomonas reinhardtii was inserted into the chromosome of Rhizobium leguminosarum bv. trifolii strain R3. The As methylation ability of the recombinant Rhizobium was tested under free living conditions and in symbiosis with red clover plants. Arsenic speciation was determined using high-performance liquid chromatography-inductively coupled plasma mass spectrometry. Under free-living conditions, CrarsM-recombinant R. leguminosarum gained the ability to methylate As(III) to methylated arsenicals, including methylarsenate [MAs(V)], dimethylarsenate [DMAs(V)] and trimethylarsine oxide [TMAs(V)O]. Red clover plants were inoculated with either control (non-recombinant) or CrarsM-recombinant R. leguminosarum and exposed to 5 or 10 μM arsenite. No methylated As species were detected in red clover plants inoculated with control R. leguminosarum. In contrast, all three methylated species were detected in both the nodules and the shoots when the recombinant Rhizobium established symbiosis with red clover, accounting for 74.7–75.1% and 29.1–42.4% of the total As in the two plant tissues, respectively. The recombinant symbiont also volatilized small amounts of As. The present study demonstrates that engineered rhizobia expressing an algal arsM gene can methylate and volatilize As, providing a proof of concept for potential future use of legume-rhizobia symbionts for As bioremediation.
登录
查看更多内容
砷污染水稻土中新型细菌介导的高效砷甲基化和挥发
影响因子：
11.4
作者：
Huang K;Chen C;Zhang J;Tang Z;Shen Q;Rosen BP;Zhao FJ
通讯作者：
Zhao FJ
影响因子：
11.4
作者：
Li, R. Y.;Stroud, J. L.;Zhao, F. J.
通讯作者：
Zhao, F. J.
具有耐热亚砷酸甲基转移酶的基因工程枯草芽孢杆菌用于砷污染的有机废物的生物修复
影响因子：
4.4
作者：
Huang, Ke;Chen, Chuan;Zhao, Fang-Jie
通讯作者：
Zhao, Fang-Jie
影响因子：
2.2
作者：
Lozano, Mauricio J.;Eugenia Salas, Maria;Lagares, Antonio
通讯作者：
Lagares, Antonio
影响因子：
7.4
作者：
Liu, Wen-Ju;Wood, B. Alan;Feldmann, Joerg
通讯作者：
Feldmann, Joerg