Simple, rapid, sensitive, and versatile SWNT-paper sensor for environmental toxin detection competitive with ELISA.
Simple, rapid, sensitive, and versatile SWNT-paper sensor for environmental toxin detection competitive with ELISA.
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DOI：
10.1021/nl902368r
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发表时间：
2009-12
期刊：
影响因子：
10.8
通讯作者：
Kotov NA
中科院分区：
文献类型：
--
作者：
Wang L;Chen W;Xu D;Shim BS;Zhu Y;Sun F;Liu L;Peng C;Jin Z;Xu C;Kotov NA
长期以来，水安全一直是许多国家和不同社区最紧迫的需求之一。尽管有许多潜在的方法来评估水的安全性，找到一种简单、快速、通用和廉价的方法来检测日常用品中的毒素仍然是一个巨大的挑战。在这项研究中，我们将单壁碳纳米管（SWNTs）浸渍多孔纤维材料（如织物和纸张）获得的复合材料的概念扩展到非常简单但高性能的生物传感器。他们利用纳米管渗透网络的电导率对纳米管-纳米管隧道间隙宽度的强烈依赖，可以潜在地满足上述常规毒素监测的所有要求。微囊藻毒素- lr （MC-LR）抗体与单壁碳纳米管一起分散。这种分散液被用来浸渍纸张，使其导电。利用纸张电导率的变化快速准确地检测水中的MC-LR。该方法线性检测范围可达10 nmol/L，非线性检测范围可达40 nmol/L。检测限为0.6 nmol/L (0.6 ng/mL)，满足世界卫生组织饮用水中MC-LR含量的最严格标准（1 ng/mL），与传统的酶联免疫吸附法检测MC-LR的检出限相当，同时分析时间大大缩短了一个数量级以上，这是实际应用中的主要障碍之一。类似的传感器制备技术也可用于其他各种快速环境传感器。
Safety of water was for a long time and still is one of the most pressing needs for many countries and different communities. Despite the fact that there are potentially many methods to evaluate water safety, finding a simple, rapid, versatile, and inexpensive method for detection of toxins in everyday items is still a great challenge. In this study, we extend the concept of composites obtained impregnation of porous fibrous materials, such as fabrics and papers, by single walled carbon-nanotubes (SWNTs) toward very simple but high-performance biosensors. They utilize the strong dependence of electrical conductivity through nanotubes percolation network on the width of nanotubes-nanotube tunneling gap and can potentially satisfy all the requirements outlined above for the routine toxin monitoring. An antibody to the microcystin-LR (MC-LR), one of the common culprits in mass poisonings, was dispersed together with SWNTs. This dispersion was used to dip-coat the paper rendering it conductive. The change in conductivity of the paper was used to sense the MC-LR in the water rapidly and accurately. The method has the linear detection range up to 10 nmol/L and non-linear detection up to 40 nmol/L. The limit of detection was found to be 0.6 nmol/L (0.6 ng/mL), which satisfies the strictest World Health Organization standard for MC-LR content in drinking water (1 ng/mL), and is comparable to the detection limit of traditional ELISA method of MC-LR detection, while drastically reducing the time of analysis by more than an order of magnitude, which is one of the major hurdles in practical applications. Similar technology of sensor preparation can also be used for a variety of other rapid environmental sensors.
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影响因子：
9.1
作者：
Shim, Bong Sup;Starkovich, John;Kotov, Nicholas
通讯作者：
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影响因子：
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影响因子：
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作者：
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作者：
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