Probiotic properties of Oxalobacter formigenes: an in vitro examination.

Probiotic properties of Oxalobacter formigenes: an in vitro examination.
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DOI:
10.1007/s00203-016-1272-y
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发表时间:
2016-12
影响因子:
2.8
通讯作者:
Knight, John
Knight, John
中科院分区:
生物学4区
文献类型:
--
作者:
Ellis, Melissa L.;Dowell, Alexander E.;Li, Xingsheng;Knight, John

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草酸杆菌是一种非致病性、革兰氏阴性、专性厌氧细菌,广泛存在于人体肠道中,能降解草酸作为其主要的能量和碳源。一项病例对照研究的结果表明,缺乏产甲氧西林杆菌的定植是复发草酸钙结石形成的危险因素。因此,定植福氏假单胞菌可能被证明是限制草酸钙结石风险的一种有效方法。然而,由于它是一种具有苛刻生长要求的厌氧菌,因此在制备成功的益生菌方面存在挑战。在这里,我们检查了一个成功的益生菌菌株的体外特性。结果表明,1群产甲酸菌OxCC13对pH和lt;5.0敏感,不含草酸,耐氧,冻干或与酸奶混合后能长时间存活。这些发现突显了该菌株对一些与益生菌菌株的制造、储存和分配相关的过程和条件的适应能力。
Oxalobacter formigenes (O. formigenes) is a nonpathogenic, Gram-negative, obligate anaerobic bacterium that commonly inhabits the human gut and degrades oxalate as its major energy and carbon source. Results from a case-controlled study suggested that lack of O. formigenes colonization is a risk factor for recurrent calcium oxalate stone formation. Hence, O. formigenes colonization may prove to be an efficacious method for limiting calcium oxalate stone risk. However, challenges exist in the preparation of O. formigenes as a successful probiotic due to it being an anaerobe with fastidious growth requirements. Here we examine in vitro properties expected of a successful probiotic strain. The data show that the Group 1 O. formigenes strain OxCC13 is sensitive to pH < 5.0, persists in the absence of oxalate, is aerotolerant, and survives for long periods when freeze-dried or mixed with yogurt. These findings highlight the resilience of this O. formigenes strain to some processes and conditions associated with the manufacture, storage and distribution of probiotic strains.
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发表时间: 2016-02
期刊: The Journal of urology
影响因子: --
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