Self-organization in high-density bacterial colonies: efficient crowd control.
Self-organization in high-density bacterial colonies: efficient crowd control.
复制标题
DOI：
10.1371/journal.pbio.0050302
复制
发表时间：
2007-10-30
期刊：
影响因子：
9.8
通讯作者：
Levchenko A
中科院分区：
文献类型：
--
作者：
Cho H;Jönsson H;Campbell K;Melke P;Williams JW;Jedynak B;Stevens AM;Groisman A;Levchenko A
细菌细胞的菌落可以表现出复杂的集体动力学，经常最终形成生物膜和其他有序的超结构。最近的研究表明，为了科普当地的环境挑战，细菌细胞可以主动寻找小腔室或空腔并在那里聚集，参与群体感应行为。通过使用一种新型的微流体装置，我们发现，在允许细胞连续逃逸的不同形状和大小的腔室内，细菌菌落可以逐渐自我组织。细胞的取向、它们的生长和集体运动的方向是相互关联的，并且由室壁和室出口的位置决定。最终高度组织化的稳定状态有利于细胞更有组织地从腔室中逃逸，并增加营养物质进入集落的机会和废物从集落中排出。使用一个计算模型，我们建议，细胞的长度可能会被优化，以最大限度地提高自组织，同时尽量减少潜在的踩踏式出口堵塞。这里描述的自组织可能是至关重要的早期阶段的组织高密度的细菌菌落填充小，物理限制的生长壁龛。这表明这种现象可以在细菌生物膜的启动和其他复杂的多细胞细菌超结构的发展中发挥关键作用，包括那些与感染性疾病有关的细菌。细菌细胞形成具有复杂组织的菌落（又名生物膜），特别是在恶劣的环境条件下。最近的研究表明，当细菌细胞寻找小空腔并以高密度填充它们时，生物膜的发展就会发生。然而，腔中的细菌可能遭受营养供应或废物去除不良，或无组织扩张导致细胞逃逸受阻。在这项研究中，我们在微流体装置中观察了大肠杆菌，该装置允许直接观察不同形状和大小的微室中细胞集落的生长和发育。结合这个实验与计算分析的菌落生长和扩张，我们表征的过程中的菌落自组织，导致在高度的相关性之间的方向的细胞取向和增长的集体细胞运动。我们还发现，这种自组织可以显着促进有效的细胞从他们居住的腔的局限性逃脱，同时改善营养物质进入殖民地内部。最后，我们建议E.大肠杆菌和其他类似细菌通常可能受到与菌落自组织相关的约束。在自然界中，细菌经常发现自己处于高密度的菌落中。一种新的微流体装置和计算分析的结合揭示了紧密堆积的细菌细胞的意想不到的自组织行为。
Colonies of bacterial cells can display complex collective dynamics, frequently culminating in the formation of biofilms and other ordered super-structures. Recent studies suggest that to cope with local environmental challenges, bacterial cells can actively seek out small chambers or cavities and assemble there, engaging in quorum sensing behavior. By using a novel microfluidic device, we showed that within chambers of distinct shapes and sizes allowing continuous cell escape, bacterial colonies can gradually self-organize. The directions of orientation of cells, their growth, and collective motion are mutually correlated and dictated by the chamber walls and locations of chamber exits. The ultimate highly organized steady state is conducive to a more-organized escape of cells from the chambers and increased access of nutrients into and evacuation of waste out of the colonies. Using a computational model, we suggest that the lengths of the cells might be optimized to maximize self-organization while minimizing the potential for stampede-like exit blockage. The self-organization described here may be crucial for the early stage of the organization of high-density bacterial colonies populating small, physically confined growth niches. It suggests that this phenomenon can play a critical role in bacterial biofilm initiation and development of other complex multicellular bacterial super-structures, including those implicated in infectious diseases. Bacterial cells form colonies with complex organization (aka biofilms), particularly in response to hostile environmental conditions. Recent studies have shown that biofilm development occurs when bacterial cells seek out small cavities and populate them at high densities. However, bacteria in cavities may suffer from poor nutrient supply or waste removal, or disorganized expansion leading to blockage of cell escape. In this study, we observed Escherichia coli in a microfluidic device that allows direct observation of the growth and development of cell colonies in microchambers of different shapes and sizes through multiple generations. Combining this experimentation with computational analysis of colony growth and expansion, we characterize a process of colony self-organization that results in a high degree of correlation between the directions of cell orientation and growth of collective cell movement. We also find that this self-organization can significantly facilitate efficient escape of cells from the confines of cavities where they reside, while improving the access of nutrients into the colony interior. Finally, we suggest that the aspect ratio of the shape of E. coli and other similar bacteria might be generally subject to a constraint related to colony self-organization. In nature, bacteria often found themselves in high-density colonies. The combination of a novel microfluidic device and computational analysis reveals an unexpected self-organization behavior of tightly packed bacterial cells.
登录
查看更多内容
影响因子：
3.2
作者：
DUNLAP, PV;GREENBERG, EP
通讯作者：
GREENBERG, EP
影响因子：
56.9
作者：
Park, S;Wolanin, PM;Austin, RH
通讯作者：
Austin, RH
影响因子：
64.8
作者：
Ozbudak, EM;Thattai, M;van Oudenaarden, A
通讯作者：
van Oudenaarden, A
影响因子：
3.2
作者：
Engelberg, D;Mimran, A;Fink, GR
通讯作者：
Fink, GR
影响因子：
64.8
作者：
Helbing, D;Farkas, I;Vicsek, T
通讯作者：
Vicsek, T