Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis.
Tannin coordinated nanozyme composite-based hybrid hydrogel eye drops for prophylactic treatment of multidrug-resistant Pseudomonas aeruginosa keratitis.
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DOI：
10.1186/s12951-022-01653-w
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发表时间：
2022-10-14
影响因子：
10.2
通讯作者：
Zhou, Qingjun
中科院分区：
文献类型：
--
作者：
Wang, Hongwei;Song, Fangying;Feng, Jing;Qi, Xia;Ma, Li;Xie, Lixin;Shi, Weiyun;Zhou, Qingjun
关键词：
绿脓杆菌感染是一种严重的急性化脓性溃疡，在短时间内几乎吞噬整个组织，并导致毁灭性的破坏。抗生素治疗是预防和治疗铜绿假单胞菌感染的常用方法。然而，它通常与严重的副作用，并发症和多药耐药性有关。因此，探索安全有效的铜绿假单胞菌感染控制方法一直是一个长期的挑战。在本文中，开发了基于单宁配位的纳米酶复合物的杂化水凝胶（TCNH），并使用小鼠角膜炎作为动物模型表征其用于预防性治疗铜绿假单胞菌和多重耐药铜绿假单胞菌感染。以乙酰化明胶溶液为原料，通过光引发自由基聚合反应制备了TCNH滴眼液，该滴眼液含有自制的单宁配位Co 3 O 4/Ag纳米酶复合物。TCNH具有良好的生物活性和过氧化物酶样活性，并具有良好的体内外生物相容性。TCNH中的纳米酶复合物似乎渗透到细菌内部，由于其固有的纳米酶催化性质，表现出显着的广谱抗菌活性。此外，TCNH滴眼液可成功应用于治疗铜绿假单胞菌和多重耐药铜绿假单胞菌角膜炎。这项研究的结果揭示了单宁协调的纳米酶复合物为基础的混合水凝胶滴眼液用于治疗感染性疾病的潜力。在线版本包含补充材料，可通过10.1186/s12951-022-01653-w获得。
Pseudomonas aeruginosa infection is a severe acute suppurative ulcer that engulfs virtually the entire tissue in a short period and leads to devastating destruction. Antibiotic therapy is a common approach for the prophylaxis and treatment of P. aeruginosa infection. However, it is often associated with serious side effects, complications, and multidrug resistance. Therefore, it has been a long-standing challenge to explore safe and effective methods for controlling P. aeruginosa infection. Herein, tannin-coordinated nanozyme composite-based hybrid hydrogels (TCNH) are developed and characterized for the prophylactic treatment of P. aeruginosa and multidrug-resistant P. aeruginosa infections using mouse keratitis as the animal model. The TCNH eye drops are constructed by photoinitiated free radical polymerization of acetylated gelatin solution containing self-synthesized tannin-coordinated Co3O4/Ag nanozyme composite. The as-prepared TCNH displays good dispersibility, peroxidase-like activity and in vitro/in vivo biocompatibility. The nanozyme composite in TCNH seems to penetrate the interior of bacteria and exhibited significant broad-spectrum antibacterial activity owing to its intrinsic and nanozymic catalytic properties. Furthermore, TCNH eye drops can be successfully applied to treat P. aeruginosa and multidrug-resistant P. aeruginosa keratitis. The findings of this study reveal the potential of tannin-coordinated nanozyme composite-based hybrid hydrogel eye drops for treating infectious diseases. The online version contains supplementary material available at 10.1186/s12951-022-01653-w.
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DOI：
10.1038/s41579-021-00649-x
发表时间：
2022-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Larsson DGJ;Flach CF
通讯作者：
Flach CF
DOI：
10.1038/s41579-020-0420-1
发表时间：
2021-01
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
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通讯作者：
Rotello VM
影响因子：
9.5
作者：
Mu, Dan;Wang, Wenshen;Zhang, Bing
通讯作者：
Zhang, Bing
影响因子：
13.6
作者：
Mu, Qianzhu;Sun, Yanfeng;Cai, Aijun
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Cai, Aijun
富含缺陷的粘附纳米酶作为有效抗生素增强细菌抑制
影响因子：
16.6
作者：
Cao, Fangfang;Zhang, Lu;Qu, Xiaogang
通讯作者：
Qu, Xiaogang