Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.
Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.
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DOI：
10.1155/2015/849769
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发表时间：
2015
影响因子：
1.8
通讯作者：
Song H
中科院分区：
文献类型：
--
作者：
Wang G;Yao S;Zhang XX;Song H
首次将2，2-二苯基-1-苦肼基-超高效液相色谱-Q-飞行时间质谱（DPPH-UPLC-Q-TOF/MS）技术用于卷柏抗氧化活性成分的筛选。筛选出9种双黄酮类化合物作为潜在的抗氧化剂。对双黄酮类化合物进行了结构鉴定，并将其分为穗花杉黄酮型、粗壮黄酮型和扁柏黄酮型三种类型。其中，bilobetin（3）和putraflavone（8）为首次从卷柏中分离得到，其它化合物包括穗花杉双黄酮（1）、罗布斯塔黄酮（2）、4′-甲氧基罗布斯塔黄酮（4）、罗汉果双黄酮A（5）、扁柏黄酮（6）、银杏黄酮（7）和常春藤黄酮（9）为首次从卷柏中分离得到。此外，9个双黄酮类化合物通过清除DPPH自由基具有良好的抗氧化活性。说明DPPH-UPLC-Q-TOF/MS对小分子具有较强的分离和鉴定能力。该方法适用于抗氧化剂的快速筛选，不需要复杂的系统和额外的仪器。
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4′-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.
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影响因子：
3.1
作者：
Tang, Dan;Li, Hui-Jun;Li, Ping
通讯作者：
Li, Ping
影响因子：
8.8
作者：
Qiu, Jiying;Chen, Leilei;Du, Fangling
通讯作者：
Du, Fangling
影响因子：
6.4
作者：
Kappler, M;Bache, M;Taubert, H
通讯作者：
Taubert, H
影响因子：
11.2
作者：
Pang X;Yi T;Yi Z;Cho SG;Qu W;Pinkaew D;Fujise K;Liu M
通讯作者：
Liu M
DOI：
10.3390/ijerph8062533
发表时间：
2011-06
影响因子：
--
作者：
Farombi EO;Owoeye O
通讯作者：
Owoeye O