Ion Activation Methods for Peptides and Proteins.
Ion Activation Methods for Peptides and Proteins.
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DOI：
10.1021/acs.analchem.5b04563
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发表时间：
2016-01-05
影响因子：
7.4
通讯作者：
Brodbelt JS
中科院分区：
文献类型：
--
作者：
Brodbelt JS
肽和蛋白质的分析以及蛋白质组学（蛋白质的大规模研究）的更大范围已经通过开发一系列通用的离子活化方法而得到了推进，这些方法促进了基于诊断片段化模式的产生的肽和蛋白质的表征。质谱仪器和样品处理方法的改进已经允许对复杂的细胞裂解物进行深入分析，从而使得除了能够全面表征翻译后修饰之外，还可以鉴定数千种蛋白质。通过串联质谱法成功地阐明了许多肽和蛋白质的一级序列，加速了支持靶向策略和定量方法的其他互补方法的发展，并催化了质谱法在相关领域（如结构生物学）的新应用。本文综述了离子活化法在生物化学、分子生物学、药物化学、生物技术和结构生物学等领域中的发展和应用。此外，解开这些活化方法的基本基础，揭示了影响离子碎片化的因素，从而提供了预测性的见解和激励新的战略，利用操纵离子解离行为的特定应用。鉴于串联质谱在蛋白质组学和结构生物学领域中发挥的关键作用，本文将重点介绍过去3年来用于分析肽和蛋白质的离子活化方法，重点介绍其新的应用。近年来，有许多优秀的综述和教程文章专注于基于质谱的蛋白质组学技术，蛋白质组学应用和特定的激活方法，因此读者可以直接阅读这些文章以提供更多的观点。1 - 24此外，最近的一篇综述特别关注蛋白质组学中的激活方法，重点是翻译后修饰的表征和用于定量的串联质谱法，7因此这些主题不在此讨论。这篇评论以一些基本的教程部分开始，提供背景信息，然后是更专业的子主题，展示了一些最近的高影响力的肽和蛋白质的激活方法的应用。
The analysis of peptides and proteins as well as the grander scope of proteomics (large scale study of proteins) has been advanced by the development of a versatile array of ion activation methods that have facilitated characterization of peptides and proteins based on production of diagnostic fragmentation patterns. Improvements of mass spectrometry instrumentation and sample processing methodologies have allowed intensive analysis of complex cell lysates, thus making it possible to identify thousands of proteins in addition to enabling comprehensive characterization of post translational modifications. The successful elucidation of the primary sequence of many peptides and proteins through tandem mass spectrometry has accelerated the development of other complementary methods that support targeted strategies and quantitative approaches and have catalyzed new applications of mass spectrometry in related fields, such as structural biology. This review will describe the development and applications of ion activation methods for peptides and proteins that have played such a critical role in the fields of biochemistry, molecular biology, medicinal chemistry, biotechnology, and structural biology. Moreover, unravelling the fundamental underpinnings of these activation methods have shed light on the factors that influence ion fragmentation upon energization, thus providing predictive insight and motivating new strategies that capitalize on manipulating ion dissociation behavior for specific applications. Given the critical role that tandem mass spectrometry has played in the field of proteomics and structural biology, this review will emphasize the ion activation methods that have been used to analyze peptides and proteins with an emphasis on new applications over the past 3 years. There are numerous excellent review and tutorial articles that have focused on mass spectrometry-based proteomics technologies, proteomic applications, and specific activation methods in recent years, and thus readers are directed to these to provide additional perspectives. 1− 24 In addition, a recent review focused specifically on activation methods in proteomics with an emphasis on characterization of posttranslational modifications and tandem mass spectrometry methods for quantitation, 7 so these topics are not covered here. This review opens with some basic tutorial sections to provide background information, followed by more specialized subtopics that demonstrate some of the more recent high impact applications of activation methods for peptides and proteins.
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影响因子：
4.4
作者：
Bauer, Manuel;Ahrne, Erik;Schmidt, Alexander
通讯作者：
Schmidt, Alexander
影响因子：
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