Photodissociation mass spectrometry: new tools for characterization of biological molecules.

Photodissociation mass spectrometry: new tools for characterization of biological molecules.
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DOI:
10.1039/c3cs60444f
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发表时间:
2014-04-21
影响因子:
46.2
通讯作者:
Brodbelt JS
Brodbelt JS
中科院分区:
化学1区
文献类型:
--
作者:
Brodbelt JS

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光解离质谱法将使用光子激活和碎片化离子的能力与通过质谱法对所得产物离子的灵敏检测相结合。由此产生的组合提供了一个通用的工具,用于表征生物分子。在过去的十年中,随着新的研究小组进入该领域并开发了许多创新应用,光解质谱的范围和广度大幅增加,这些应用说明了光解产生丰富的碎片模式,选择性地切割键,并基于生色团的掺入靶向特定分子的能力。本文综述了近十年来光解离质谱的主要进展,特别强调了它在生物分子中的应用。
Photodissociation mass spectrometry combines the ability to activate and fragment ions using photons with the sensitive detection of the resulting product ions by mass spectrometry. The resulting combination affords a versatile tool for characterization of biological molecules. The scope and breadth of photodissociation mass spectrometry have increased substantially over the past decade as new research groups have entered the field and developed a number of innovative applications that illustrate the ability of photodissociation to produce rich fragmentation patterns, to cleave bonds selectively, and to target specific molecules based on incorporation of chromophores. This review focuses on many of the key developments in photodissociation mass spectrometry over the past decade with a particular emphasis on its applications to biological molecules.
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