Spatial and Temporal Analysis of Host Cells in Tracheal Graft Implantation.
Spatial and Temporal Analysis of Host Cells in Tracheal Graft Implantation.
复制标题
气管移植物植入过程中宿主细胞的时空分析。
DOI：
10.1002/lary.28781
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Chiang T
中科院分区：
文献类型：
--
作者：
Danielson A;Liu L;Shontz KM;Syed H;Dharmadhikari S;Reynolds SD;Breuer CK;Chiang T
理想的气管替代物应该是与宿主基因相同的活体移植物，从而避免免疫抑制的需要。我们已经开发了一种小鼠同种气管移植模型，其结果是长期存活，没有移植物狭窄或延迟愈合。为了了解宿主细胞如何促进气管移植整合，我们量化了移植物和原生气管中宿主细胞的数量。气管移植，气管置换，再生医学，动物模型。从雌性C57BL/6小鼠身上获得气管移植物，并将其原位移植到同基因雄性受体体内。在移植后第14天、第45天和第90天实施安乐死。分别对宿主和移植物气管进行外植，并进行组织学分析。用荧光原位杂交法定量雄性宿主细胞，用免疫荧光法定量巨噬细胞。最早时间点（14天）在中移植物中发现宿主来源细胞的证据。宿主来源的细胞在移植物中短暂增加，主要出现在粘膜下层。到第90天，宿主来源的细胞数量下降到第14天的水平。在所有时间点均观察到宿主和移植物组织的巨噬细胞浸润，在第90天最大。气管移植物的整合是通过亚急性短暂性宿主细胞浸润发生的，本质上主要是炎症性的。宿主细胞对移植物上皮的贡献是有限的。这些数据表明，创造活的、非免疫原性的气管移植物可以作为长段气管缺陷的可行解决方案。
The ideal trachea replacement would be a living graft that is genetically identical to the host, avoiding the need for immunosuppression. We have developed a mouse model of syngeneic tracheal transplant that results in long-term survival without graft stenosis or delayed healing. To understand how host cells contribute to tracheal transplant integration, we quantified the populations of host cells in the graft and native trachea following implant. Tracheal transplant, tracheal replacement, regenerative medicine, animal model. Tracheal grafts were obtained from female C57BL/6 mice and orthotopically transplanted into syngeneic male recipients. Cohorts were euthanized on day 14, day 45, and day 90 post-transplantation. Host and graft tracheas were explanted and analyzed by histology. Male host cells were quantified using fluorescence in situ hybridization, and macrophages were quantified with immunofluorescence. Evidence of host-derived cells was found in the midgraft at the earliest time point (14 days). Host-derived cells transiently increased in the graft on day 45 and were predominantly found in the submucosa. By day 90, the population of host-derived cells population declined to a similar level on day 14. Macrophage infiltration of host and graft tissue was observed at all time points and was greatest on day 90. Tracheal graft integration occurs by way of subacute transient host-cell infiltration and is primarily inflammatory in nature. Host-cell contribution to the graft epithelium is limited. These data indicate that creation of living, nonimmunogenic tracheal graft could serve as a viable solution for long-segment tracheal defects.
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影响因子：
0.9
作者：
Konoeda, C.;Koinuma, D.;Morishita, Y.;Sano, A.;Nagayama, K.;Motomura, N.;Kakimi, K.;Miyazono, K.;Nakajima, J.;Nicolis, M. R.;Murakawa, T.
通讯作者：
Murakawa, T.
影响因子：
6.2
作者：
Genden, EM;Boros, P;Mayer, L
通讯作者：
Mayer, L
影响因子：
8.8
作者：
Neuringer, IP;Aris, RM;Randell, SH
通讯作者：
Randell, SH
原位和异位气管移植模型在闭塞性细支气管炎研究中的应用
影响因子：
1.5
作者：
Fan, Kai;Qiao, Xin-Wei;Jiang, Ke
通讯作者：
Jiang, Ke
DOI：
10.1177/0003489416656646
发表时间：
2016-11
期刊：
The Annals of otology, rhinology, and laryngology
影响因子：
--
作者：
Chiang T;Pepper V;Best C;Onwuka E;Breuer CK
通讯作者：
Breuer CK