Age associated oxidative damage in lymphocytes.
Age associated oxidative damage in lymphocytes.
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DOI：
10.4161/oxim.3.4.12860
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发表时间：
2010-07
影响因子：
--
通讯作者：
Roy S
中科院分区：
文献类型：
--
作者：
Gautam N;Das S;Kar Mahapatra S;Chakraborty SP;Kundu PK;Roy S
淋巴细胞是一种重要的免疫细胞，在获得性免疫系统中起着重要的作用，因此，在免疫衰老中可能起着关键作用。据报道，氧化应激在老年受试者中增加，可能是由于随着衰老和抗氧化防御能力降低而产生的自由基不受控制。本研究旨在评估健康个体淋巴细胞中脂质-蛋白质损伤水平和抗氧化状态，以确定氧化损伤与衰老过程之间的相关性。每个年龄组（11-20岁、21-30岁、31-40岁、41-50岁和51-60岁）随机选择20名健康个体。由执业医师抽取血液样品，并从血液样品中分离淋巴细胞。检测淋巴细胞丙二醛（MDA）、蛋白质羰基（PC）含量，以判断淋巴细胞脂质和蛋白质损伤程度。超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽和谷胱甘肽依赖性酶的测定，以评估淋巴细胞的抗氧化状态。增加的MDA和PC水平强烈支持老年受试者比年轻受试者增加的氧化损伤。结果表明，在衰老过程中，淋巴细胞氧化和抗氧化系统的平衡发生了变化，有利于加速氧化损伤。因此，淋巴细胞中的氧化应激可能在衰老中特别感兴趣，并且可能在免疫衰老中起重要作用。
Lymphocytes are an important immunological cell and have been played a significant role in acquired immune system; hence, may play in pivotal role in immunosenescence. Oxidative stress has been reported to increase in elderly subjects, possibly arising from an uncontrolled production of free radicals with aging and decreased antioxidant defenses. This study was aimed to evaluate the level of lipid-protein damage and antioxidant status in lymphocytes of healthy individuals to correlate between oxidative damage with the aging process. Twenty healthy individuals of each age group (11–20; 21–30; 31–40; 41–50; and 51–60 years) were selected randomly. Blood samples were drawn by medical practitioner and lymphocytes were isolated from blood samples. Malondialdehyde (MDA), protein carbonyls (PC) level were evaluated to determine the lipid and protein damage in lymphocytes. Superoxide dismutase (SOD), catalase (CAT), glutathione and glutathione dependent enzymes were estimated to evaluate the antioxidant status in the lymphocytes. Increased MDA and PC levels strongly support the increased oxidative damage in elderly subject than young subjects. The results indicated that, balance of oxidant and antioxidant systems in lymphocytes shifts in favor of accelerated oxidative damage during aging. Thus oxidative stress in lymphocytes may particular interest in aging and may play important role in immunosenescence.
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影响因子：
3.9
作者：
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通讯作者：
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