Epidermal aquaporin-3 is increased in the cutaneous burn wound.
Epidermal aquaporin-3 is increased in the cutaneous burn wound.
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DOI：
10.1016/j.burns.2014.10.033
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Milner SM
中科院分区：
文献类型：
--
作者：
Sebastian R;Chau E;Fillmore P;Matthews J;Price LA;Sidhaye V;Milner SM
水通道蛋白 (AQP) 是跨膜蛋白家族，可跨细胞膜运输水和甘油等小溶质。它是跨细胞水流的介质，通过响应渗透梯度的变化促进水运输，在维持细胞内/外液体稳态中发挥重要作用。在皮肤中，AQP 允许快速、可调节和选择性的水渗透，并已被证明在皮肤水合作用、细胞增殖、迁移、免疫和伤口愈合中发挥作用。然而，AQP-3在皮肤烧伤创面中的表达尚未阐明。我们试图评估烧伤患者中 AQP-3 的表达。受伤后大约 3-7 天，从 7 名患者 (n = 21) 的烧伤创面中心、烧伤创面边缘和移植供体部位采集新鲜的全层活检样本。使用 AQP-3 特异性抗体对固定的石蜡包埋切片进行染色，并通过免疫荧光进行检查。对新鲜样品进行处理，通过蛋白质印迹分析定量 AQP-3 蛋白表达。烧伤创面的中心部分显示表皮和真皮遭到破坏，且不存在 AQP-3。沿着表皮结构被破坏的烧伤创面边缘，存在强烈的 AQP-3 染色。蛋白质印迹分析表明，与未烧伤的皮肤（对照）相比，烧伤创面边缘的染色更深。蛋白质定量显示，与未烧伤皮肤 (2.1 ± 0.28，N = 7，*p < 0.05) 相比，烧伤创面边缘有大量 AQP-3 表达 (3.6 ± 0.34)。烧伤创面中心无AQP-3表达。受伤后烧伤创面中 AQP-3 的表达增加。虽然其在伤口愈合中的作用已被确定，但我们首次报告了皮肤烧伤对 AQP-3 表达的影响。我们的数据为确定其在烧伤创面中的功能作用提供了第一步。我们假设 AQP3 靶向疗法的开发可能会改善烧伤创面的愈合。
Aquaporins (AQP) are a family of transmembrane proteins that transport water and small solutes such as glycerol across cell membranes. It is a mediator of transcellular water flow and plays an important role in maintaining intra/extracellular fluid homeostasis by facilitating water transport in response to changing osmotic gradients. In the skin, AQPs permit rapid, regulated, and selective water permeability and have been demonstrated to play a role in skin hydration, cell proliferation, migration, immunity, and wound healing. However, the expression of AQP-3 in the cutaneous burn wound has never been elucidated. We sought to assess the expression of AQP-3 in patients with burn wounds. A fresh full thickness biopsy sample was taken from the center of the burn wound, the burn wound edge, and the graft donor site in 7 patients (n = 21), approximately 3–7 days post injury. Fixed, paraffin embedded sections were stained using AQP-3 specific antibody and examined by immunofluorescence. Fresh samples were processed to quantify AQP-3 protein expression with Western blot analysis. The central portion of the burn wound revealed destruction of the epidermis and dermis with no AQP-3 present. Along the burn wound edge where the epidermal architecture was disrupted, there was robust AQP-3 staining. Western blot analysis demonstrated deeper staining along the burn wound edge compared to unburned skin (control). Quantification of the protein shows a significant amount of AQP-3 expression along the burn wound edge (3.6 ± 0.34) compared to unburned skin (2.1 ± 0.28, N = 7, *p < 0.05). There is no AQP-3 expression in the burn wound center. AQP-3 expression is increased in the burn wound following injury. While its role in wound healing has been defined, we report for the first time the effect of cutaneous burns on AQP-3 expression. Our data provides the first step in determining its functional role in burn wounds. We hypothesize that development of AQP3 targeted therapies may improve burn wound healing.
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烧伤伤口中缺氧和缺氧诱导因子。
DOI：
10.1111/j.1524-475x.2010.00656.x
发表时间：
2011-03
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Xing D;Liu L;Marti GP;Zhang X;Reinblatt M;Milner SM;Harmon JW
通讯作者：
Harmon JW
影响因子：
4.8
作者：
Hara, M;Ma, TH;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
4.6
作者：
Wang, G. F.;Dong, C. L.;Bai, C. X.
通讯作者：
Bai, C. X.
影响因子：
8.7
作者：
Romani N;Clausen BE;Stoitzner P
通讯作者：
Stoitzner P
影响因子：
4
作者：
Shaw, Tanya J.;Martin, Paul
通讯作者：
Martin, Paul