Formation of the apical pole of epithelial (Madin-Darby canine kidney) cells: polarity of an apical protein is independent of tight junctions while segregation of a basolateral marker requires cell-cell interactions.
Formation of the apical pole of epithelial (Madin-Darby canine kidney) cells: polarity of an apical protein is independent of tight junctions while segregation of a basolateral marker requires cell-cell interactions.
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DOI：
10.1083/jcb.104.4.905
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发表时间：
1987-04
期刊：
影响因子：
--
通讯作者：
Rodriguez-Boulan E
中科院分区：
文献类型：
--
作者：
Vega-Salas DE;Salas PJ;Gundersen D;Rodriguez-Boulan E
使用半薄（约0.5微米）冷冻切片和单克隆抗体探针的半定量免疫荧光追踪Madin-Darby犬肾细胞顶侧（184 kD）和底侧（63 kD）质膜蛋白极性发展的时间过程。在正常培养基和1-5 μ M Ca 2+中，184-kD蛋白在最初的24小时内高度极化至顶端，这导致细胞分布良好，呈圆顶状，缺乏紧密连接和其他侧膜相互作用。相比之下，基底外侧63-kD膜蛋白只有在正常Ca 2+浓度下孵育超过72小时后才能完全极性化，这比形成紧密连接所需的时间（约18小时）长得多，并且在1-5 μ M Ca 2+中不能形成。这些结果表明，独立于紧密连接，如自我聚集或特定的相互作用与亚膜细胞骨架的结构域内的限制机制，参与区域化的至少一些上皮细胞质膜蛋白。这些机制的全面运作取决于正常细胞-细胞相互作用的存在下，在基底外侧63-kD抗原的情况下，但不是在顶端184-kD蛋白的情况下。
The time course of development of polarity of an apical (184-kD) and a basolateral (63-kD) plasma membrane protein of Madin-Darby canine kidney cells was followed using semiquantitative immunofluorescence on semithin (approximately 0.5-micron) frozen sections and monoclonal antibody probes. The 184-kD protein became highly polarized to the apical pole within the initial 24 h both in normal medium and in 1-5 microM Ca2+, which results in well-spread, dome-shaped cells, lacking tight junctions and other lateral membrane interactions. In contrast, the basolateral 63-kD membrane protein developed full polarity only after incubation in normal Ca2+ concentrations for greater than 72 h, a time much longer than that required for the formation of tight junctions (approximately 18 h) and failed to polarize in 1-5 microM Ca2+. These results demonstrate that intradomain restriction mechanisms independent of tight junctions, such as self-aggregation or specific interactions with the submembrane cytoskeleton, participate in the regionalization of at least some epithelial plasma membrane proteins. The full operation of these mechanisms depends on the presence of normal cell-cell interactions in the case of the basolateral 63-kD antigen but not in the case of the apical 184-kD protein.
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DOI：
10.1073/pnas.79.15.4672
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HALL, HG;FARSON, DA;BISSELL, MJ
通讯作者：
BISSELL, MJ
DOI：
10.1073/pnas.81.1.224
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FRADET, Y;CORDONCARDO, C;OLD, LJ
通讯作者：
OLD, LJ
涉及建立上皮遮挡屏障的蛋白质的功能分析：识别紫外线蛋白样多肽。
影响因子：
7.8
作者：
GUMBINER, B;SIMONS, K
通讯作者：
SIMONS, K
影响因子：
56.9
作者：
FISHER, KA;STOECKENIUS, W
通讯作者：
STOECKENIUS, W
影响因子：
11.4
作者：
BALCAROVASTANDER, J;PFEIFFER, SE;SIMONS, K
通讯作者：
SIMONS, K