Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans.
Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans.
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Coniothyrium minitans 黑色素相关基因 CmMR1 的功能分析
DOI：
10.3389/fmicb.2018.02658
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发表时间：
2018
影响因子：
5.2
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Luo C;Zhao H;Yang X;Qiang C;Cheng J;Xie J;Chen T;Jiang D;Fu Y
小孢子虫（Coniothyrium minitans）是一种菌核寄生虫，已被研究用于农作物菌核病的商业防治。此前，我们获得了一个T-DNA插入突变体ZS-1TN24363，该突变体在条件作用期间不产生黑色素。为了了解C. minitans中黑色素的功能，我们克隆了被T-DNA插入破坏的基因，发现该基因被称为CmMR1，编码了一个推定的1011个氨基酸的蛋白，这是转录因子mr的同源物，全长CmMR1包含3167 bp，有3个外显子和2个内含子。为了证实被破坏的基因是导致突变体缺乏黑色素的原因，我们对CmMR1进行了破坏，获得了三个靶向敲除突变体。生物学分析表明，靶向敲除突变体的表型与T-DNA插入突变体相似。此外，基因互补证实CmMR1负责突变表型。CmMR1突变体的破坏不影响小丝蛾菌丝的生长、分生和寄生，但ROS积累增加，对紫外线的耐受性显著降低。本研究结果可从分子水平上加深对黑素在小锥虫生态学中的认识。
Coniothyrium minitans is a sclerotial parasite, which has been investigated for commercial control of crop diseases caused by Sclerotinia sclerotiorum. Previously, we obtained a T-DNA insertional mutant, ZS-1TN24363, which did not produce melanin during conidiation. To understand the function of melanin in C. minitans, we cloned the gene that was disrupted by the T-DNA insertion, and found that this gene, called CmMR1, encoded a putative protein of 1,011 amino acids, which is a homolog of the transcription factor MR. Full-length CmMR1 contains 3,167 bp, with three exons and two introns. To confirm that the disrupted gene is responsible for the melanin-deficiency of the mutant, CmMR1 was disrupted and three targeted knockout mutants were obtained. Biological assays showed that the phenotype of the targeted knockout mutants was similar to that of the T-DNA insertional mutant. Furthermore, gene complementation confirmed that CmMR1 is responsible for the mutant phenotype. CmMR1 disruption did not affect hyphal growth, conidiation, and parasitization of C. minitans, however, the ROS accumulation increased and tolerance to UV light decreased significantly in the mutants. Our result may enhance the understanding of melanin in the ecology of C. minitans on molecular level.
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影响因子：
5.2
作者：
Zhang X;Liu X;Zhao Y;Cheng J;Xie J;Fu Y;Jiang D;Chen T
通讯作者：
Chen T
Nox 复合物信号和 MAPK 级联途径是交联的，对于真菌寄生虫 Coniothyrium minitans 的致病性和分生孢子至关重要
影响因子：
4.6
作者：
Wei W;Zhu W;Cheng J;Xie J;Jiang D;Li G;Chen W;Fu Y
通讯作者：
Fu Y
影响因子：
2.1
作者：
Li, MX;Gong, XY;Hou, MS
通讯作者：
Hou, MS
影响因子：
2.8
作者：
CAMPBELL, WA
通讯作者：
CAMPBELL, WA
影响因子：
3.5
作者：
CHUMLEY, FG;VALENT, B
通讯作者：
VALENT, B