Plasma membrane-targeted PIN proteins drive shoot development in a moss.
Plasma membrane-targeted PIN proteins drive shoot development in a moss.
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DOI：
10.1016/j.cub.2014.09.054
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发表时间：
2014-12-01
期刊：
影响因子：
--
通讯作者：
Harrison CJ
中科院分区：
文献类型：
--
作者：
Bennett TA;Liu MM;Aoyama T;Bierfreund NM;Braun M;Coudert Y;Dennis RJ;O'Connor D;Wang XY;White CD;Decker EL;Reski R;Harrison CJ
植物体平面在发育过程中由分生生长顶端的活动产生，并在进化过程中的配子体（n）和孢子体（2n）阶段独立辐射。虽然生长素和PIN家族外排载体的细胞间运输是开花植物孢子体芽发育的主要调节因子，但在陆地植物中PIN功能的保守程度和调节芽配子体芽发育的机制在很大程度上是未知的。我们已经发现用外源生长素和生长素运输抑制剂处理藓类小立碗藓的配子体芽会破坏顶端功能和叶发育。两种质膜靶向PIN蛋白在叶枝中表达，pin突变体类似于用生长素或生长素转运抑制剂处理的植物。PIN介导的生长素运输调节苔藓配子体顶端细胞功能、叶起始、叶形状和芽向性。pin突变孢子体有时会分枝，复制以前只在化石记录和罕见的天然苔藓变种中看到的表型。我们的研究结果表明，PIN介导的生长素运输是一个古老的，保守的调节芽发育。PIN蛋白在苔藓中具有极性质膜定位，立碗藓中PIN介导的生长素运输驱动立碗藓中配子体芽的发育，PIN介导的生长素运输抑制立碗藓孢子体中的分枝。班尼特等人显示PIN介导的生长素运输调节苔藓两个生命周期阶段的芽发育，并确定PIN蛋白在植物体计划进化中的潜在作用。
Plant body plans arise by the activity of meristematic growing tips during development and radiated independently in the gametophyte (n) and sporophyte (2n) stages of the life cycle during evolution. Although auxin and its intercellular transport by PIN family efflux carriers are primary regulators of sporophytic shoot development in flowering plants, the extent of conservation in PIN function within the land plants and the mechanisms regulating bryophyte gametophytic shoot development are largely unknown. We have found that treating gametophytic shoots of the moss Physcomitrella patens with exogenous auxins and auxin transport inhibitors disrupts apical function and leaf development. Two plasma membrane-targeted PIN proteins are expressed in leafy shoots, and pin mutants resemble plants treated with auxins or auxin transport inhibitors. PIN-mediated auxin transport regulates apical cell function, leaf initiation, leaf shape, and shoot tropisms in moss gametophytes. pin mutant sporophytes are sometimes branched, reproducing a phenotype only previously seen in the fossil record and in rare natural moss variants. Our results show that PIN-mediated auxin transport is an ancient, conserved regulator of shoot development. PIN proteins have polar plasma membrane localizations in the moss Physcomitrella PIN-mediated auxin transport drives gametophytic shoot development in Physcomitrella PIN-mediated auxin transport suppresses branching in Physcomitrella sporophytes Shooting systems have undergone 450 million years of independent evolution in flowering plant sporophytes and bryophyte gametophytes. Bennett et al. show that PIN-mediated auxin transport regulates shoot development in both life cycle stages in a moss and identify potential roles for PIN proteins in the evolution of plant body plans.
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影响因子：
11.6
作者：
Ganguly, Anindya;Park, Minho;Cho, Hyung-Taeg
通讯作者：
Cho, Hyung-Taeg
DOI：
10.1002/9781118305881.ch1
发表时间：
2013-01-01
期刊：
EVOLUTION OF PLANT FORM
影响因子：
--
作者：
Doyle, James A.
通讯作者：
Doyle, James A.
DOI：
10.1002/9781118305881.ch2
发表时间：
2013-01-01
期刊：
EVOLUTION OF PLANT FORM
影响因子：
--
作者：
Goffinet, Bernard;Buck, William R.
通讯作者：
Buck, William R.
影响因子：
6.2
作者：
Bierfreund, NM;Reski, R;Decker, EL
通讯作者：
Decker, EL
影响因子：
4.6
作者：
Eklund, D. Magnus;Thelander, Mattias;Sundberg, Eva
通讯作者：
Sundberg, Eva