Focal BOLD fMRI changes in bicuculline-induced tonic-clonic seizures in the rat.
Focal BOLD fMRI changes in bicuculline-induced tonic-clonic seizures in the rat.
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DOI：
10.1016/j.neuroimage.2010.01.006
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发表时间：
2010-04-15
期刊：
影响因子：
5.7
通讯作者：
Blumenfeld, Hal
中科院分区：
文献类型：
--
作者：
DeSalvo, Matthew N.;Schridde, Ulrich;Mishra, Asht M.;Motelow, Joshua E.;Purcaro, Michael J.;Danielson, Nathan;Bai, Xiaoxiao;Hyder, Fahmeed;Blumenfeld, Hal
全身强直阵挛性发作引起大脑皮层和皮层下结构的广泛生理变化。使用9.4 T的血氧水平依赖性（BOLD）功能磁共振成像（fMRI）和脑电图（EEG），可以以高时空分辨率表征这些变化。我们研究了麻醉Wistar大鼠在荷包牡丹碱诱导的强直-阵挛性癫痫发作期间BOLD的变化。全身注射GABAA受体拮抗剂荷包牡丹碱后，EEG表现为高幅、高频多棘波放电，随后为低频阵发性阵发性发作，平均发作持续时间为349 s。我们的目的是表征BOLD信号在发作前、发作和发作后的空间定位、方向和时间变化。使用叠加在高分辨率解剖图像上的成对BOLD信号t图对癫痫发作进行组分析。然后利用感兴趣的数量进行区域分析，以量化BOLD时间进程。在发作前期，我们发现局灶性BOLD增加在特定区域的躯体感觉皮层（S1，S2）和丘脑几秒钟前癫痫发作。在癫痫发作期间，我们观察到BOLD在皮质、脑干和丘脑中增加，而在海马中BOLD减少。最大的发作BOLD增加保持在躯体感觉皮层的局灶性区域，显示发作前增加。在发作后期间，我们观察到广泛的BOLD降低。这些发现支持了一个模型，其中“全身性”强直阵挛性癫痫发作开始于电描记癫痫发作前的局灶性变化，在癫痫发作期间进展为不均匀的变化，可能揭示了人类类似癫痫发作的病因学和病理生理学。
Generalized tonic-clonic seizures cause widespread physiological changes throughout the cerebral cortex and subcortical structures in the brain. Using combined blood oxygen level dependent (BOLD) functional magnetic resonance imaging (fMRI) at 9.4 T and electroencephalography (EEG) these changes can be characterized with high spatiotemporal resolution. We studied BOLD changes in anesthetized Wistar rats during bicuculline-induced tonic-clonic seizures. Bicuculline, a GABAA receptor antagonist, was injected systemically and seizure activity was observed on EEG as high amplitude, high-frequency polyspike discharges followed by clonic paroxysmal activity of lower frequency, with mean electrographic seizure duration of 349 s. Our aim was to characterize the spatial localization, direction, and timing of BOLD signal changes during the pre-ictal, ictal and post-ictal periods. Group analysis was performed across seizures using paired t-maps of BOLD signal superimposed on high resolution anatomical images. Regional analysis was then performed using volumes of interest to quantify BOLD timecourses. In the pre-ictal period we found focal BOLD increases in specific areas of somatosensory cortex (S1, S2) and thalamus several seconds before seizure onset. During seizures we observed BOLD increases in cortex, brainstem and thalamus and BOLD decreases in the hippocampus. The largest ictal BOLD increases remained in the focal regions of somatosensory cortex showing pre-ictal increases. During the post-ictal period we observed widespread BOLD decreases. These findings support a model in which “generalized” tonic-clonic seizures begin with focal changes before electrographic seizure onset, which progress to non-uniform changes during seizures, possibly shedding light on the etiology and pathophysiology of similar seizures in humans.
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影响因子：
3.3
作者：
GRUETTER, R
通讯作者：
GRUETTER, R
影响因子：
2.9
作者：
Klein, JP;Khera, DS;Blumenfeld, H
通讯作者：
Blumenfeld, H
DOI：
10.1111/j.1749-6632.1986.tb51260.x
发表时间：
1986-03-14
影响因子：
5.2
作者：
ACKERMANN, RF;ENGEL, J;BAXTER, L
通讯作者：
BAXTER, L
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5.6
作者：
André, V;Henry, D;Nehlig, A
通讯作者：
Nehlig, A
DOI：
10.1097/01.wcb.0000131669.02027.3e
发表时间：
2004-09-01
影响因子：
6.3
作者：
Nersesyan, H;Herman, P;Blumenfeld, H
通讯作者：
Blumenfeld, H