Expression of the metastasis‐associated mts1 gene during mouse development
Expression of the metastasis‐associated mts1 gene during mouse development
复制标题
小鼠发育过程中转移相关 mts1 基因的表达
作者：
J. Klingelhöfer;N. Ambartsumian;E. Lukanidin
mts1基因是S100家族的成员，在不同的转移性肿瘤细胞系中特异性表达。转染某些非转移细胞系后，Mts1 可以诱导转移表型。 Mts1 蛋白可以与非肌肉肌球蛋白相互作用，表明 Mts1 在细胞运动中发挥作用。为了了解该基因的功能，我们研究了小鼠发育过程中mts1 mRNA和蛋白的体内表达。性交后 12.5 至 18.5 天 (dpc)，mRNA 和蛋白质均以高浓度存在于各种中胚层来源的发育胚胎组织中。我们通过巨噬细胞特异性抗体的双重免疫染色发现，Mts1蛋白在整个胚胎间充质的胎儿巨噬细胞以及定植发育中的淋巴和非淋巴器官的巨噬细胞中高表达。此外，我们发现mts1在间充质组织的分化和形态发生过程中表达，例如指尖周围的间充质、膀胱上皮下的间充质、鼻囊原基和皮肤之间的间充质以及正在发育的毛发和牙囊的真皮乳头中的间充质。在骨骼发育过程中，Mts1 在侵袭性间充质细胞和破骨细胞中表达。这里提出的结果表明，Mts1 在巨噬细胞分化和功能期间的小鼠发育中发挥重要作用，并且可能参与与间充质形态发生相关的不同过程，包括间充质-上皮相互作用、组织重塑和侵袭。开发。动态。 1997；210：87-95。 © 1997 Wiley-Liss, Inc.
The mts1 gene, a member of the S100 family, is specifically expressed in different metastatic tumor cell lines. After transfection in some nonmetastatic cell lines Mts1 can induce a metastatic phenotype. Mts1 protein can interact with non‐muscle myosin, indicating that Mts1 plays a role in cell motility. In order to understand the function of this gene, we studied the expression of the mts1 mRNA and protein in vivo during mouse development. Both mRNA and protein were present in high concentrations from 12.5 to 18.5 days post coitum (dpc) in a variety of developing embryonic tissue of mesodermal origin. We found by double immunostaining with a macrophage‐specific antibody that Mts1 protein was highly expressed in fetal macrophages throughout the embryonic mesenchyme and in macrophages colonizing developing lymphatic and non‐lymphatic organs. Moreover, we found mts1 expression during differentiation and morphogenesis of mesenchymal tissues such as the mesenchyme surrounding the tips of digits, the mesenchyme underlying the epithelium of the bladder, and the mesenchyme between the primordia of the nasal capsule and the skin as well as in the developing dermal papilla of hair and tooth follicle. In developing bone, Mts1 was expressed in invasive mesenchymal cells and in osteoclasts. The results presented here suggest that Mts1 plays an important role in mouse development during differentiation and function of macrophages and might be involved in different processes associated with mesenchymal morphogenesis including mesenchymal‐epithelial interaction, tissue remodeling, and invasion. Dev. Dyn. 1997;210:87–95. © 1997 Wiley‐Liss, Inc.
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影响因子：
8
作者：
Heide L. Ford;Sayeeda Zain
通讯作者：
Heide L. Ford;Sayeeda Zain
影响因子：
4.4
作者：
M. Ho;T. Springer
通讯作者：
M. Ho;T. Springer
影响因子：
4.6
作者：
Richard A. Lang;Marc Lustig;Fritz Francois;Meredith Sellinger;Heide Plesken
通讯作者：
Richard A. Lang;Marc Lustig;Fritz Francois;Meredith Sellinger;Heide Plesken
胚胎和胎儿造血：概述。
DOI：
--
发表时间：
1991
期刊：
Blood cells
影响因子：
--
作者：
Tavassoli,M
通讯作者：
Tavassoli,M