Specific histamine binding activity of a new lipocalin from Hyalomma asiaticum (Ixodidae) and therapeutic effects on allergic asthma in mice.
Specific histamine binding activity of a new lipocalin from Hyalomma asiaticum (Ixodidae) and therapeutic effects on allergic asthma in mice.
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亚洲透明蜱（Ixodidae）新型脂质运载蛋白的特异性组胺结合活性及其对小鼠过敏性哮喘的治疗作用
DOI：
10.1186/s13071-016-1790-0
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发表时间：
2016-09-17
影响因子：
3.2
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Wang Y;Li Z;Zhou Y;Cao J;Zhang H;Gong H;Zhou J
脂质运载蛋白由蜱唾液腺分泌，是干扰宿主免疫反应的重要策略。分泌大量脂质运载蛋白，但大多数这些蛋白质的功能尚不清楚。在这里，我们报告了一种具有特定组胺结合能力的新脂质运载蛋白，该蛋白是从蜱亚洲透明蜱的唾液腺中分离出来的。通过RACE获得Ha24基因的全长cDNA，并在大肠杆菌中表达Ha24基因；蛋白质纯化和小鼠免疫后，针对重组蛋白产生了特异性多克隆抗体 (PcAb)。采用逆转录PCR（RT-PCR）、定量PCR（Q-PCR）、间接免疫荧光抗体（IFA）测定和蛋白质印迹法检测蜱中天然Ha24的存在。为了确认 rHa24 的组胺结合能力，通过抑制小鼠过敏性哮喘，完成了体外 (ELISA) 和体内组胺结合测定。 Ha24 由 681 个碱基编码，包含 227 个氨基酸，分子量为 23.3 kDa。通过鉴定蜱中的天然 Ha24 证实了摄食蜱唾液腺中的丰富表达。体外和体内组胺结合测定的结果表明，rHa24 以剂量依赖性方式与组胺特异性结合，并且可以缓解小鼠过敏性哮喘。 Ha24 是一种新型蜱脂质运载蛋白，具有特异性组胺结合活性，可以缓解宿主炎症反应。
Lipocalin proteins are secreted by tick salivary glands as an important strategy to interfere with the immune response of hosts. A large number of lipocalins are secreted, but the functions of most of these proteins are unclear. Here, we report a new lipocalin protein with particular histamine binding capacity, which was isolated from the salivary glands of the tick Hyalomma asiaticum. The full length cDNA of the Ha24 gene was obtained by RACE, and Ha24 gene was expressed in E. coli; after protein purification and mice immunizations, specific Polyclonal antibodies (PcAb) were created in response to the recombinant protein. Reverse transcription PCR (RT-PCR), Quantitative PCR (Q-PCR), indirect immunofluorescence antibody (IFA) assay and western blot were used to detect the existence of native Ha24 in ticks. To confirm the histamine-binding capacity of rHa24, a histamine-binding assay was completed in vitro (ELISA) and in vivo by inhibition of allergic asthma in mice. Ha24 is coded by 681 bases, contains 227 amino acids, and has a molecular weight of 23.3 kDa. Abundant expression in the salivary glands of feeding ticks was confirmed by the identification of native Ha24 in ticks. The results of a histamine binding assay both in vitro and in vivo demonstrated that rHa24 binds specifically with histamine in a dose-dependent manner, and can provide relief from allergic asthma in mice. Ha24 is a new tick lipocalin with specific histamine binding activity that can provide relief from host inflammation response.
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影响因子：
16.6
作者：
Gulia-Nuss M;Nuss AB;Meyer JM;Sonenshine DE;Roe RM;Waterhouse RM;Sattelle DB;de la Fuente J;Ribeiro JM;Megy K;Thimmapuram J;Miller JR;Walenz BP;Koren S;Hostetler JB;Thiagarajan M;Joardar VS;Hannick LI;Bidwell S;Hammond MP;Young S;Zeng Q;Abrudan JL;Almeida FC;Ayllón N;Bhide K;Bissinger BW;Bonzon-Kulichenko E;Buckingham SD;Caffrey DR;Caimano MJ;Croset V;Driscoll T;Gilbert D;Gillespie JJ;Giraldo-Calderón GI;Grabowski JM;Jiang D;Khalil SMS;Kim D;Kocan KM;Koči J;Kuhn RJ;Kurtti TJ;Lees K;Lang EG;Kennedy RC;Kwon H;Perera R;Qi Y;Radolf JD;Sakamoto JM;Sánchez-Gracia A;Severo MS;Silverman N;Šimo L;Tojo M;Tornador C;Van Zee JP;Vázquez J;Vieira FG;Villar M;Wespiser AR;Yang Y;Zhu J;Arensburger P;Pietrantonio PV;Barker SC;Shao R;Zdobnov EM;Hauser F;Grimmelikhuijzen CJP;Park Y;Rozas J;Benton R;Pedra JHF;Nelson DR;Unger MF;Tubio JMC;Tu Z;Robertson HM;Shumway M;Sutton G;Wortman JR;Lawson D;Wikel SK;Nene VM;Fraser CM;Collins FH;Birren B;Nelson KE;Caler E;Hill CA
通讯作者：
Hill CA
影响因子：
3.8
作者：
Mans, BJ;Neitz, AWH
通讯作者：
Neitz, AWH
影响因子：
6.7
作者：
Preston SG;Majtán J;Kouremenou C;Rysnik O;Burger LF;Cabezas Cruz A;Chiong Guzman M;Nunn MA;Paesen GC;Nuttall PA;Austyn JM
通讯作者：
Austyn JM
影响因子：
15
作者：
Syme NR;Dennis C;Bronowska A;Paesen GC;Homans SW
通讯作者：
Homans SW
DOI：
10.1016/s0006-291x(05)81256-2
发表时间：
1991-10-15
影响因子：
3.1
作者：
FLOWER, DR;NORTH, ACT;ATTWOOD, TK
通讯作者：
ATTWOOD, TK