The translational oscillation in oocyte and early embryo development.
The translational oscillation in oocyte and early embryo development.
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DOI：
10.1093/nar/gkad996
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发表时间：
2023-12-11
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
--
作者：
翻译对于发育至关重要，因为卵母细胞和早期胚胎中的转录是沉默的。为了阐明卵母细胞和早期胚胎减数分裂和连续两次有丝分裂期间的翻译变化，我们进行了全基因组翻译组分析。所获得的数据显示，M期特异性的关键翻译起始和延伸轴的显著和均匀活化。虽然全球蛋白质合成减少M期，翻译起始和延长活动增加，在一个统一的波动方式，导致通过mTOR 1/4F/eEF 2轴的翻译调控的质的变化。总的来说，我们已经发现了一个高度动态和振荡模式的翻译重编程，有助于特定的mRNA的翻译调控与不同模式的多聚体占用/翻译，这是卵母细胞和胚胎发育能力的重要。我们的研究结果提供了新的见解，在卵母细胞减数分裂以及前两个胚胎有丝分裂的基因表达调控，并显示如何时间翻译可以优化。这项研究是全面分析分子机制的第一步，这些机制不仅控制早期发育过程中的翻译，而且还调节卵母细胞向胚胎过渡和胚胎基因组激活中的发育相关网络。
Translation is critical for development as transcription in the oocyte and early embryo is silenced. To illustrate the translational changes during meiosis and consecutive two mitoses of the oocyte and early embryo, we performed a genome-wide translatome analysis. Acquired data showed significant and uniform activation of key translational initiation and elongation axes specific to M-phases. Although global protein synthesis decreases in M-phases, translation initiation and elongation activity increases in a uniformly fluctuating manner, leading to qualitative changes in translation regulation via the mTOR1/4F/eEF2 axis. Overall, we have uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to the translational regulation of specific mRNAs with different modes of polysomal occupancy/translation that are important for oocyte and embryo developmental competence. Our results provide new insights into the regulation of gene expression during oocyte meiosis as well as the first two embryonic mitoses and show how temporal translation can be optimized. This study is the first step towards a comprehensive analysis of the molecular mechanisms that not only control translation during early development, but also regulate translation-related networks employed in the oocyte-to-embryo transition and embryonic genome activation.
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影响因子：
2.3
作者：
Cooper KF;Strich R
通讯作者：
Strich R
影响因子：
2.7
作者：
HASHIMOTO, N;KISHIMOTO, T
通讯作者：
KISHIMOTO, T
影响因子：
5.6
作者：
Gholami A;Minai-Tehrani D;Mahdizadeh SJ;Saenz-Mendez P;Eriksson LA
通讯作者：
Eriksson LA
影响因子：
16
作者：
Park, Jong-Eun;Yi, Hyerim;Kim, V. Narry
通讯作者：
Kim, V. Narry
影响因子：
4.7
作者：
Dong M;Chen J;Deng Y;Zhang D;Dong L;Sun D
通讯作者：
Sun D