Acridine orange leukocyte fluorography in mice.
Acridine orange leukocyte fluorography in mice.
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DOI：
10.1016/j.exer.2013.12.002
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发表时间：
2014-03
影响因子：
3.4
通讯作者：
Ambati, Balamurali K.
中科院分区：
文献类型：
--
作者：
Cahoon, Judd M.;Olson, Paul R.;Nielson, Spencer;Miya, Tadashi R.;Bankhead, Peter;McGeown, J. Graham;Curtis, Timothy M.;Ambati, Balamurali K.
可以在眼睛中获得患者血管和神经的同时非侵入性可视化。视网膜血管系统是许多视网膜病的靶点。炎症，很容易表现为白细胞粘附到内皮衬里，是许多视网膜病变的关键病理生理机制，使其成为疾病研究的一个有价值的和普遍存在的目标。白细胞荧光造影术在过去的二十年中得到了广泛的应用，然而，荧光标记物、可视化技术和记录方法在不同的研究中有所不同。缺乏关于白细胞荧光造影的详细方案文件，加上研究之间缺乏一致性，导致视网膜中白细胞转运（速度、粘附性、外渗）的标准缺乏。在这里，我们给出了一个方便的方法，使用吖啶橙子（AO）和市售的扫描激光检眼镜（SLO，HRA-OCT光谱）查看白细胞在小鼠视网膜的行为的详细描述。将正常小鼠与患有急性和慢性炎症的小鼠进行比较。这种方法可以很容易地在许多研究实验室采用。
Simultaneous non-invasive visualization of blood vessels and nerves in patients can be obtained in the eye. The retinal vasculature is a target of many retinopathies. Inflammation, readily manifest by leukocyte adhesion to the endothelial lining, is a key pathophysiological mechanism of many retinopathies, making it a valuable and ubiquitous target for disease research. Leukocyte fluorography has been extensively used in the past twenty years; however, fluorescent markers, visualization techniques, and recording methods have differed between studies. The lack of detailed protocol papers regarding leukocyte fluorography, coupled with lack of uniformity between studies, has led to a paucity of standards for leukocyte transit (velocity, adherence, extravasation) in the retina. Here, we give a detailed description of a convenient method using acridine orange (AO) and a commercially available scanning laser ophthalmoscope (SLO, HRA-OCT Spectralis) to view leukocyte behavior in the mouse retina. Normal mice are compared to mice with acute and chronic inflammation. This method can be readily adopted in many research labs.
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影响因子：
3.4
作者：
Xu, HP;Manivannan, A;Crane, IJ
通讯作者：
Crane, IJ
影响因子：
--
作者：
JANSSEN, GHGW;TANGELDER, GJ;RENEMAN, RS
通讯作者：
RENEMAN, RS
影响因子：
6
作者：
Joussen, AM;Murata, T;Adamis, AP
通讯作者：
Adamis, AP
影响因子：
4.4
作者：
Xu, HP;Forrester, JV;Crane, IJ
通讯作者：
Crane, IJ
影响因子：
4.4
作者：
Miyahara, S;Kiryu, J;Yoshimura, N
通讯作者：
Yoshimura, N