New human hepatocellular carcinoma (HCC) cell line with highly metastatic potential (MHCC97) and its expressions of the factors associated with metastasis.
New human hepatocellular carcinoma (HCC) cell line with highly metastatic potential (MHCC97) and its expressions of the factors associated with metastasis.
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DOI：
10.1038/sj.bjc.6690769
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发表时间：
1999-11
影响因子：
8.8
通讯作者：
Xue Q
中科院分区：
文献类型：
--
作者：
Tian J;Tang ZY;Ye SL;Liu YK;Lin ZY;Chen J;Xue Q
采用体外细胞培养和裸鼠体内生长交替的方法，从人肝癌裸鼠皮下移植瘤模型（LCI-D20）建立了一株新的高转移性人肝癌细胞系。该系命名为MHCC 97，已培养18个月，并传代培养90多次。染色体核型分析表明该细胞系为人源细胞。细胞生长为紧密集落（成簇）或单层片，群体倍增时间约为31 h，形态学上显示典型的恶性上皮细胞，甲胎蛋白（AFP）阳性。细胞DNA含量的流式细胞仪分析显示非整倍体模式，其指数为1.5相比，正常人外周血淋巴细胞。G-和C-显带技术的核型分析显示，所有细胞的染色体数目和结构的异常。细胞系MHCC 97的染色体数目范围为59 - 65，众数为60和61。至少有两个共同的染色体标记i（1 q）和der（4）t（4;？）（4pter→q35：：？），在所有细胞中均存在，并且在所有细胞中也存在Y染色体缺失。将其接种裸鼠皮下和肝内形成异种移植瘤，并发现肺转移灶。原位接种肺转移率为100%。逆转录聚合酶链反应产物显示整合素α5和β1、尿激酶型纤溶酶原激活物受体（uPAR）、血管内皮生长因子和nm 23-H1 mRNA在MHCC 97细胞系中呈阳性表达。c-Met、uPAR在移植瘤和肺转移灶中呈强阳性表达，而整合素α5、β1在移植瘤中呈阳性表达，在肺转移灶中呈阴性表达。E-cadherin和P53在移植瘤和转移灶中均不表达。PCR产物HBsAg和HBxAg均为阳性。细胞系MHCC 97仍然保留了原始肿瘤的一些特征。MHCC 97细胞系的建立将有助于肝癌转移机制的研究。© 1999癌症研究运动
A new human hepatocellular carcinoma (HCC) cell line with a highly metastatic potential was established from subcutaneous xenograft of a metastatic model of human HCC in nude mice (LCI-D20) by means of alternating cell culture in vitro and growth in nude mice. The line, designated MHCC97, has been cultivated for 18 months and subcultured for more than 90 passages. The line was showed to be of human origin by karyotype analysis. The cells were either grown as compact colonies (in clusters) or as a monolayered sheet with about 31 h of population-doubling time, exhibited typical malignant epithelial in morphology and were positive for α-fetoprotein (AFP). Flow cytometric analysis of the cell DNA content showed an aneuploid pattern, and its index was 1.5 as compared to that of normal human peripheral blood lymphocytes. Karyotypic analyses of G- and C-banding techniques revealed that all cells presented chromosome abnormalities in number and structure. The number of cell line MHCC97 chromosome ranged from 59 to 65 with a modal number of 60 and 61. At least two common chromosome markers, i(1q) and der(4)t(4;?)(4pter→q35::?), were present in all cells, and deletion of Y chromosome also occurred in all cells. The subcutaneous and intrahepatic xenografts were formed and metastatic lesions in lungs were found after the cells were inoculated into nude mice. The rate of metastasis to lungs was 100% using orthotopic inoculation. Reverse transcription polymerase chain reaction products revealed positive expressions of integrin α5 and β1, urokinase type plasminogen activator receptor (uPAR), vascular endothelial growth factor and nm23-H1 mRNAs of cell line MHCC97. Immunostaining of c-Met, uPAR showed strongly positive in both subcutaneous xenografts and lung metastatic lesions; while positive in xenografts and negative in metastatic lesions for integrin α5, β1. E-cadherin and P53 was not expressed either in xenograft or in the metastatic lesions. PCR products of HBsAg and HBxAg were both positive. The cell line MHCC97 still retained some characteristic features of original tumour. Establishment of cell line MHCC97 should be beneficial to the studies of HCC metastatic mechanisms. © 1999 Cancer Research Campaign
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影响因子：
64.5
作者：
HSU, TY;MOROY, T;BUENDIA, MA
通讯作者：
BUENDIA, MA
影响因子：
3.8
作者：
Hambly, RJ;Double, JA;Bibby, MC
通讯作者：
Bibby, MC
影响因子：
64.8
作者：
FOUREL, G;TREPO, C;BUENDIA, MA
通讯作者：
BUENDIA, MA
影响因子：
--
作者：
BARDI, G;JOHANSSON, B;MITELMAN, F
通讯作者：
MITELMAN, F
DOI：
10.1093/jnci/51.5.1417
发表时间：
1973-01-01
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
GIARD, DJ;AARONSON, SA;PARKS, WP
通讯作者：
PARKS, WP