Accurate processing of human pre-rRNA in vitro.
Accurate processing of human pre-rRNA in vitro.
复制标题
人 rRNA 前体的体外精确加工。
DOI：
10.1128/mcb.9.10.4422-4431.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Nilsen,TW
中科院分区：
文献类型：
--
作者：
Hannon,GJ;Maroney,PA;Branch,A;Benenfield,BJ;Robertson,HD;Nilsen,TW
We report here that the mature 5′ terminus of human 18S rRNA is generated in vitro by a two-step processing reaction. In the first step, SP6 transcripts were specifically cleaved in HeLa cell nucleolar extract at three positions near the external transcribed spacer (ETS)-18S boundary. Of these cleavage sites, two were major and the other was minor. RNase T1fingerprint and secondary nuclease analyses placed the two major cleavage sites 3 and 8 bases upstream from the mature 5′ end of 18S rRNA and the minor cleavage site 1 base into the 18S sequence. All three cleavages yielded 5′-hydroxyl, 2′-3′-cyclic phosphate termini and were 5′ of adenosine residues in the sequence UACCU, which was repeated three times near the ETS-18S boundary. In the second step, the initial cleavage product containing 3 bases of ETS was converted to an RNA with a 5′ terminus identical to that of mature 18S RNA by an activity found in HeLa cell cytoplasmic extracts.
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真菌和植物中小核 RNA 的高度复杂性。
影响因子：
5.6
作者：
D. Tollervey
通讯作者：
D. Tollervey
酵母中核糖体前体核糖核酸的细胞质成熟。
影响因子：
4.8
作者：
S. Udem;J. R. Warner
通讯作者：
J. R. Warner
影响因子：
14.9
作者：
Savkur, RS;Olson, MOJ
通讯作者：
Olson, MOJ
酿酒酵母小 GTP 酶 Gsp1p/Ran 参与 7S 至 5.8S rRNA 的 3 加工以及 35S pre-rRNA 切除的 5-A0 片段的降解，这两者均由外泌体进行
影响因子：
3.3
作者：
N. Suzuki;E. Noguchi;N. Nakashima;M. Oki;T. Ohba;A. Tartakoff;M. Ohishi;T. Nishimoto
通讯作者：
T. Nishimoto
酵母中 RNA 合成的调控 III
DOI：
--
发表时间：
1979
期刊：
Molecular and General Genetics MGG
影响因子：
--
作者：
S. Elliott;C. McLaughlin
通讯作者：
C. McLaughlin