A hybrid lipid membrane coating "shape-locks" silver nanoparticles to prevent surface oxidation and silver ion dissolution.
A hybrid lipid membrane coating "shape-locks" silver nanoparticles to prevent surface oxidation and silver ion dissolution.
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不经历表面氧化和Ag+离子溶解的稳定的银纳米颗粒（AgNP）的受控合成仍然是一个主要挑战。在这里，描述了稳健的混合脂质涂覆的AgNP的合成，所述混合脂质涂覆的AgNP由1-α-磷脂酰胆碱（PC）膜组成，所述1-α-磷脂酰胆碱（PC）膜由化学计量量的长链疏水性硫醇和油酸钠（SOA）作为疏水性结合配偶体锚定，所述长链疏水性硫醇和油酸钠（SOA）不经历表面氧化和Ag+离子溶解。紫外-可见（UV-Vis）光谱，透射电子显微镜（TEM），电感耦合等离子体质谱（ICP-MS）表明，在强氧化剂，如氰化钾（KCN）的存在下，混合脂质包被的银纳米粒子是稳定的，不进行表面氧化，即使在膜去稳定的表面活性剂的存在下。UV-Vis研究表明，各种尺寸和形状的混合脂质涂覆的AgNP的稳定性取决于硫醇烃链的长度，并且可以按照如下增加稳定性的顺序排列：丙硫醇（PT）<己硫醇（HT）<癸硫醇（DT）。UV-Vis和ICP-MS研究表明，混合脂质包被的AgNP的大小或形状没有变化，这证实了当放置在强氧化剂、氯化物、硫醇和低pH值的存在下时，AgNP不经历表面氧化和Ag+离子溶解。超过21天的长期稳定性研究表明，混合脂质包被的AgNP不释放Ag+离子，并且更稳定。总体而言，这些研究表明，纳米材料的混合膜封装是一种可行的方法，用于将AgNP稳定在“形状锁定”形式，该形式不能经历表面氧化、Ag+离子释放、老化或形状转换。更重要的是，这种设计策略是一种简单的方法来合成和稳定的银纳米粒子的各种生物医学和商业应用，其中Ag+离子的释放和毒性是一个问题。有了强大的和屏蔽的银纳米粒子，研究人员现在可以评估和关联银纳米粒子的物理特征如何影响毒性，而没有样品中存在的Ag+离子的混杂因素。这种设计策略还提供了一个机会，其中可以调节膜组成以控制Ag+离子的释放速率，从而优化抗微生物活性。混合脂质膜屏蔽和保护银纳米颗粒（AgNP）免受表面氧化、Ag+离子释放、老化和形状转化。
The controlled synthesis of stable silver nanoparticles (AgNPs), that do not undergo surface oxidation and Ag+ ion dissolution, continues to be a major challenge. Here the synthesis of robust hybrid lipid-coated AgNPs, comprised of l-α-phosphatidylcholine (PC) membranes anchored by a stoichiometric amount of long-chained hydrophobic thiols and sodium oleate (SOA) as hydrophobic binding partners, that do not undergo surface oxidation and Ag+ ion dissolution, is described. UV-Visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and inductively coupled plasma mass spectrometry (ICP-MS) demonstrate that in the presence of strong oxidants, such as potassium cyanide (KCN), the hybrid lipid-coated AgNPs are stable and do not undergo surface oxidation even in the presence of membrane destabilizing surfactants. UV-Vis studies show that the stability of hybrid lipid-coated AgNPs of various sizes and shapes is dependent on the length of the thiol hydrocarbon chain and can be ranked in the order of increasing stability as follows: propanethiol (PT) < hexanethiol (HT) ≤ decanethiol (DT). UV-Vis and ICP-MS studies show that the hybrid lipid-coated AgNPs do not change in size or shape confirming that the AgNPs do not undergo surface oxidation and Ag+ ion dissolution when placed in the presence of strong oxidants, chlorides, thiols, and low pH. Long-term stability studies, over 21 days, show that the hybrid lipid-coated AgNPs do not release Ag+ ions and are more stable. Overall, these studies demonstrate hybrid membrane encapsulation of nanomaterials is a viable method for stabilizing AgNPs in a “shape-locked” form that is unable to undergo surface oxidation, Ag+ ion release, aging, or shape conversion. More importantly, this design strategy is a simple approach to the synthesis and stabilization of AgNPs for a variety of biomedical and commercial applications where Ag+ ion release and toxicity is a concern. With robust and shielded AgNPs, investigators can now evaluate and correlate how the physical features of AgNPs influence toxicity without the confounding factor of Ag+ ions present in samples. This design strategy also provides an opportunity where the membrane composition can be tuned to control the release rate of Ag+ ions for optimizing antimicrobial activity. Hybrid lipid membranes shields and protects silver nanoparticles (AgNPs) from surface oxidation, Ag+ ion release, aging, and shape conversion.
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DOI：
10.3390/nano8090681
发表时间：
2018-08-31
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Burdușel AC;Gherasim O;Grumezescu AM;Mogoantă L;Ficai A;Andronescu E
通讯作者：
Andronescu E
影响因子：
6
作者：
Banasiuk, Rafal;Krychowiak, Marta;Krolicka, Aleksandra
通讯作者：
Krolicka, Aleksandra
DOI：
10.1016/s0927-7757(01)00854-8
发表时间：
2002-02-04
影响因子：
5.2
作者：
Chen, YH;Yeh, CS
通讯作者：
Yeh, CS
影响因子：
2.9
作者：
DLUHY, RA;CAMERON, DG;MENDELSOHN, R
通讯作者：
MENDELSOHN, R
DOI：
10.1016/j.jpba.2019.112970
发表时间：
2020-01-30
影响因子：
3.4
作者：
Aygun, Aysenur;Ozdemir, Sadin;Sen, Fatih
通讯作者：
Sen, Fatih