Mulberry leaf polysaccharide supplementation contributes to enhancing the respiratory mucosal barrier immune response in Newcastle disease virus-vaccinated chicks.
Mulberry leaf polysaccharide supplementation contributes to enhancing the respiratory mucosal barrier immune response in Newcastle disease virus-vaccinated chicks.
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补充桑叶多糖有助于增强新城疫病毒疫苗接种雏鸡的呼吸道粘膜屏障免疫反应
DOI：
10.1016/j.psj.2020.11.039
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发表时间：
2021-03
期刊：
影响因子：
4.4
通讯作者：
Jiang C
中科院分区：
文献类型：
--
作者：
Chen X;Yang H;Jia J;Chen Y;Wang J;Chen H;Jiang C
尽管全球疫苗接种覆盖率很高，但由于抗体水平低，纽卡斯尔病（ND）仍然是家禽生产商的持续威胁。鉴于呼吸道黏膜是纽卡斯尔病毒（NDV）免疫的重要部位，增强呼吸道黏膜免疫可能有助于控制ND。我们的前期研究表明桑葚多糖（MLP）具有很好的免疫调节作用，但其对雏鸡呼吸道免疫的影响尚不清楚。在这项研究中，我们评估了MLP激活呼吸道粘膜免疫的潜力，并揭示了MLP作为ND疫苗免疫增强剂的可能机制。将雏鸡随机分为5组：空白对照组、免疫对照组（VC）、低、中、高剂量MLP（MLP-L、MLP-M、MLP-H）组（n = 30）。体液和细胞介导的免疫应答的血清结果显示，与VC组相比，MLP-M组的NDV血凝抑制抗体滴度、IgG和伊加抗体水平以及T淋巴细胞群显著增加。结果验证还表明MLP-M组中气管抗体介导的免疫和粘膜免疫应答显著增加。此外，TLR 7的上调揭示了一种可能的机制。我们的研究结果提供了证据，考虑MLP作为一个潜在的粘膜疫苗佐剂候选人对鸡ND。
Despite high global vaccination coverage, Newcastle disease (ND) remains a constant threat to poultry producers owing to low antibody levels. Given the respiratory mucosa is the important site for Newcastle disease virus (NDV) vaccination, enhancing respiratory mucosal immunity may help control ND. Our previous study showed that mulberry leaf polysaccharide (MLP) is very promising in delivering a robust balanced immune response, but the effects of it on respiratory immunity in chicks are unknown. In this study, we evaluated the potential of MLP to activate respiratory mucosal immunity and revealed the possible mechanism of MLP as an immunopotentiator for ND vaccines. Chicks were randomly divided into 5 groups: blank control, vaccination control (VC), and low-, middle-, and high-dose MLP (MLP-L, MLP-M, and MLP-H) (n = 30). The serum results of humoral and cell-mediated immune responses showed significant increases in NDV hemagglutination inhibition antibody titer, IgG and IgA antibody levels, and the T-lymphocyte population in the MLP-M group compared with the VC group. Validation of results also indicated remarkable increases in tracheal antibody-mediated immunity and a mucosal immune response in the MLP-M group. Furthermore, the upregulation of TLR7 revealed a possible mechanism. Our findings provided evidence to consider MLP as a potential mucosal vaccine adjuvant candidate against ND in chickens.
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影响因子：
2.9
作者：
Kapczynski, Darrell R.;Afonso, Claudio L.;Miller, Patti J.
通讯作者：
Miller, Patti J.
影响因子：
3.7
作者：
Barjesteh, Neda;Taha-Abdelaziz, Khaled;Sharif, Shayan
通讯作者：
Sharif, Shayan
影响因子：
13.6
作者：
Jiménez-Dalmaroni MJ;Gerswhin ME;Adamopoulos IE
通讯作者：
Adamopoulos IE
影响因子：
3.7
作者：
Grueber CE;Wallis GP;Jamieson IG
通讯作者：
Jamieson IG
影响因子：
3.6
作者：
Arias Á;Vicario M;Bernardo D;Olalla JM;Fortea M;Montalban-Arques A;Martínez-Fernández P;González-Castro AM;Mota-Huertas T;Arias-González L;Lucendo AJ
通讯作者：
Lucendo AJ