A method for surface E-selectin site density determination
A method for surface E-selectin site density determination
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一种表面E-选择素位点密度测定方法
DOI：
10.1080/21553769.2015.1033654
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发表时间：
2015-04
影响因子：
--
通讯作者：
Ying Fang
中科院分区：
文献类型：
--
作者：
Jinhe Zhang;Bing Huang;Yingchen Ling;Ying Fang
E-选择素介导的白细胞粘附在内皮细胞上是炎症反应中的关键事件。在平行板流动室中，E-选择素在基底表面上的位点密度测定是理解E-选择素诱导细胞粘附机制的主要步骤。迄今为止，荧光法由于灵敏度较弱而无法测量低分子密度表面，而传统的125 I放射性碘标记法只能检测试管中溶液的放射性。在这项研究中，测量低站点密度表面，一种新的方法是通过结合125 I放射性碘标记与GE Infinia豪克耶4 ECT。位点密度与E-选择素孵育浓度之间的关系呈饱和曲线，在低位点密度范围内呈线性相关。当聚苯乙烯表面与浓度为10和40 ng/ml的E-选择素孵育时，位点密度分别为9和140个位点/μm2。 在此密度下，HL-60细胞可以在流动室的基底上束缚和滚动。在这项研究中开发的放射性碘标记方法是一种理想的检测方法，即使是低密度的表面，因为它的高灵敏度，这将提供更好地了解细胞粘附使用流动室。
E-selectin-mediated leukocyte adhesion on endothelial cells is a critical event in the inflammatory response. Site density determination of E-selectin on the substrate surface is the primary step in understanding the mechanism of E-selectin-induced cell adhesion in a parallel plate flow chamber. So far, the fluorescent method cannot measure low molecular density surfaces because of its weak sensitivity, and the traditional 125I radioiodination method can only detect the radioactivity of a solution in a tube. In this study, to measure low site density surfaces, a new method was constructed by combining 125I radioiodination with a GE Infinia Hawkeye 4 ECT. A saturation curve was obtained for the relationship between site densities and incubated concentrations of E-selectin, and a linear correlation was found within the range of low site density. Site densities were 9 and 140 sites/µm2 when polystyrene surfaces were incubated with E-selectin concentrations of 10 and 40 ng/ml, respectively. Under these densities, HL-60 cells could tether and roll on the substrates of the flow chamber. The radioiodination method developed in this study is an ideal detection method for even low-density surfaces because of its high sensitivity, which will provide better understanding of cell adhesion using a flow chamber.
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影响因子：
5.4
作者：
Kayani, Sadaf;Ahmad, Mushtaq;Yaseen, Ghulam
通讯作者：
Yaseen, Ghulam
影响因子：
--
作者：
Khaskheli, Abid Ali;Talpur, Farah N.;Afridi, Hassan Imran
通讯作者：
Afridi, Hassan Imran
力依赖性键解离通过 E-选择素控制 HL-60 细胞的滚动
影响因子：
3.7
作者：
Li, Quhuan;Fang, Ying;Wu, Jianhua
通讯作者：
Wu, Jianhua
影响因子：
--
作者：
Tabassum N;Rafique U;Balkhair KS;Ashraf MA
通讯作者：
Ashraf MA
影响因子：
1.1
作者：
Qureshi, Tahira;Memon, Najma;Ashraf, Muhammad Aqeel
通讯作者：
Ashraf, Muhammad Aqeel