Characterization of sodium carboxymethyl cellulose by comprehensive two-dimensional liquid chromatography.
Characterization of sodium carboxymethyl cellulose by comprehensive two-dimensional liquid chromatography.
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全二维液相色谱法表征羧甲基纤维素钠
DOI：
10.1016/j.carbpol.2015.04.011
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发表时间：
2015
影响因子：
11.2
通讯作者：
Wolfgang Radke
中科院分区：
文献类型：
--
作者：
Maryia Shakun;Thomas Heinze;Wolfgang Radke
以低分子质量Avicel纤维素（Avicel样品）和高分子质量棉绒（BWL样品）为原料，合成了两个系列的羧甲基纤维素钠（NaCMC），平均取代度（DS）在0.45 ~ 1.55之间。采用在线二维液相色谱法对样品进行表征，第一维采用梯度液相吸附色谱法，第二维采用粒径排除色谱法。该方法可以同时测定单个样品的化学成分（DS）和摩尔质量分布。此外，还得到了梯度色谱中洗脱量与摩尔质量的关系。正如预期的那样，与高分子质量的BWL样品相比，在低分子质量的NaCMC样品中发现了摩尔质量对梯度洗脱体积影响更大的证据。最后证明了该方法对化学成分和摩尔质量均不均匀的共混物同时分离的适用性。这样的混合物不能有效地分离单独的SEC或梯度色谱，也不是简单地结合两种方法的结果。只有完整的二维色谱图才能揭示这种混合物的复杂性，因为它揭示了摩尔质量和化学成分之间的相关性。
Two series of sodium carboxymethyl celluloses (NaCMC) with average degrees of substitution (DS) ranging from 0.45 to 1.55 were synthesized from low molecular mass Avicel cellulose (Avicel samples) and from high molecular mass cotton linters (BWL samples). The samples were characterized by online two-dimensional liquid chromatography using gradient liquid adsorption chromatography in the first and size exclusion chromatography (SEC) in the second dimension. This method allows the simultaneous determination of the chemical composition (DS) and the molar mass distribution within the individual samples. Moreover information was obtained on the dependence of the elution volume in gradient chromatography on molar mass. As expected, evidence for a stronger influence of molar mass on gradient elution volume was found for the low molecular mass NaCMC as compared to the high molecular mass BWL samples. Finally the applicability of the method for the simultaneous separation of blends heterogeneous with respect to chemical composition (DS) and molar mass was demonstrated. Such blends cannot be efficiently separated by either SEC or gradient chromatography alone, nor by simply combining the results of both methods. Only the complete two-dimensional chromatogram can reveal the complexity of such blends, since it reveals the correlations between molar mass and chemical composition.
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梯度色谱法测定未降解羧甲基纤维素钠的DS分布
影响因子：
11.2
作者：
Maryia Shakun;Thomas Heinze;Wolfgang Radke
通讯作者：
Wolfgang Radke
使用二维色谱根据 DS 和摩尔质量表征醋酸纤维素。
影响因子：
11.2
作者：
H. O. Ghareeb;W. Radke
通讯作者：
W. Radke
影响因子：
11.2
作者：
Eremeeva, TE;Bykova, TO
通讯作者：
Bykova, TO
通过尺寸排阻色谱法和梯度洗脱反相沉淀色谱法对共聚物进行二维分析
DOI：
--
发表时间：
1984
期刊：
影响因子：
--
作者：
G. Glöckner;J. D. V. Berg;N. Meijerink;T. Scholte;R. Koningsveld
通讯作者：
R. Koningsveld
影响因子：
4.1
作者：
Raust, Jacques-Antoine;Bruell, Adele;Pasch, Harald
通讯作者：
Pasch, Harald