HPLC determination of isoflavone levels in osage orange from the Midwest and southern United States.
HPLC determination of isoflavone levels in osage orange from the Midwest and southern United States.
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DOI：
10.1021/jf400954m
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发表时间：
2013-07-17
影响因子：
6.1
通讯作者：
Abourashed EA
中科院分区：
文献类型：
--
作者：
Darji K;Miglis C;Wardlow A;Abourashed EA
Maclura pomifera树的果实是一种可持续的重要植物黄酮，osajin和pomiferin的来源。建立了一种反相高效液相色谱法，用于鉴别高胆固醇含量的欧鼠尾草橙子样品，并确定了样品前处理的最佳条件。分析运行时间为8 min，流速为1 mL/min，使用乙腈/H2O（0.1%甲酸）梯度，UV峰检测为274 nm。对该方法的专属性、准确度、精密度以及检测限和定量限（LOD/LOQ）进行了验证。该方法被应用于确定的水平，osajin和pomiferin的提取物制备的不同样品的osage橙子生长在美国中西部和南部。结果表明，不同的变量，如样品制备，地理位置和生长阶段，对分析样品中的osajin和pomiferin的水平的影响。
The fruit of Maclura pomifera tree is a sustainable source for the pharmacologically interesting isoflavones, osajin and pomiferin. A reversed-phase HPLC method was developed to identify osage orange samples with high isoflavone content and to determine the optimum conditions for sample preparation. Analytical run time was 8 min at a flow rate of 1 mL/min using a gradient of acetonitrile in H2O (0.1% formic acid) and UV peak detection at 274 nm. The method was validated for specificity, accuracy, precision, and limits of detection and quantitation (LOD/LOQ). The method was applied to determine the levels of osajin and pomiferin in extracts prepared from different samples of osage orange growing in the United States Midwest and South. Results demonstrated the effect of different variables, such as sample preparation, geographical location, and growth stage, on the levels of osajin and pomiferin in analyzed samples.
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影响因子：
3.8
作者：
DELLEMONACHE, G;DE ROSA, MC;BOTTA, B
通讯作者：
BOTTA, B
DOI：
10.1080/01635581.2011.535959
发表时间：
2011-01-01
影响因子：
2.9
作者：
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通讯作者：
Roufogalis, Basil D.
影响因子：
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作者：
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通讯作者：
Yesilada, Erdem
影响因子：
4.3
作者：
Orhan, I.;Senol, F. S.;Mokry, P.
通讯作者：
Mokry, P.
影响因子：
2
作者：
SCHALL, ED;QUACKENBUSH, FW
通讯作者：
QUACKENBUSH, FW