Comparative Proteomic Analyses of Poorly Motile Swamp Buffalo Spermatozoa Reveal Low Energy Metabolism and Deficiencies in Motility-Related Proteins.
Comparative Proteomic Analyses of Poorly Motile Swamp Buffalo Spermatozoa Reveal Low Energy Metabolism and Deficiencies in Motility-Related Proteins.
复制标题
运动性差的沼泽布法罗精子的比较蛋白质组学分析揭示了运动相关蛋白的低能量代谢和缺陷。
DOI：
10.3390/ani12131706
复制
发表时间：
2022-07-01
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
对正常和活动性差的布法罗精子的蛋白质组学分析表明，活动性差的布法罗精子的形成与能量代谢效率低下、精子鱼精蛋白减少、缺乏运动相关蛋白以及尾部结构蛋白的变化有关。本研究结果为揭示布法罗精子活力低下的分子机制提供了线索。哺乳动物精子活力的获得是附睾精子成熟的主要指标，有助于确保受精。精子活力差会使精子细胞无法到达受精部位，导致受精失败。为了研究正常和活动性差的布法罗精子的蛋白质组学特征，采用液相色谱串联质谱与串联质谱靶向相结合的策略。结果，在运动性差的精子中鉴定出145个差异表达蛋白（DEP）（倍数变化> 1.5），包括52个上调蛋白和93个下调蛋白。上调的DEPs主要参与细胞的形态发生和分化调节。下调的DEPs参与转运、氧化还原、精子运动、cAMP代谢调节和DNA甲基化调节。PRM1和AKAP3的mRNA和蛋白水平在运动能力差的精子中较低，而SDC2、TEKT3和IDH1的表达与运动能力无关，表明它们的蛋白变化受转录或翻译的影响。这些蛋白质表达的变化表明，运动性差的布法罗精子的形成反映了能量代谢的低效率，精子精蛋白的减少，运动相关蛋白的缺乏，以及尾部结构蛋白的变化。此类蛋白质可能是活动能力较差的精子的生物标志物。这些结果阐明了与精子运动障碍相关的分子机制，并为寻找这些途径的分子标记物提供了线索。
Proteomic analysis of normal and poorly motile buffalo sperm indicates that the formation of poorly motile buffalo sperm is associated with inefficient energy metabolism, decreased sperm protamine proteins, a lack of motility-related proteins, and changes in tail structural proteins. The results provide clues for finding molecular mechanisms for poor motility of buffalo sperm. The acquisition of mammalian sperm motility is a main indicator of epididymal sperm maturation and helps ensure fertilization. Poor sperm motility will prevent sperm cells from reaching the fertilization site, resulting in fertilization failure. To investigate the proteomic profiling of normal and poorly motile buffalo spermatozoa, a strategy applying liquid chromatography tandem mass spectrometry combined with tandem mass targeting was used. As a result, 145 differentially expressed proteins (DEPs) were identified in poorly motile spermatozoa (fold change > 1.5), including 52 upregulated and 93 downregulated proteins. The upregulated DEPs were mainly involved in morphogenesis and regulation of cell differentiation. The downregulated DEPs were involved with transport, oxidation-reduction, sperm motility, regulation of cAMP metabolism and regulation of DNA methylation. The mRNA and protein levels of PRM1 and AKAP3 were lower in poorly motile spermatozoa, while the expressions of SDC2, TEKT3 and IDH1 were not correlated with motility, indicating that their protein changes were affected by transcription or translation. Such changes in the expression of these proteins suggest that the formation of poorly motile buffalo spermatozoa reflects a low efficiency of energy metabolism, decreases in sperm protamine proteins, deficiencies in motility-related proteins, and variations in tail structural proteins. Such proteins could be biomarkers of poorly motile spermatozoa. These results illustrate some of the molecular mechanisms associated with poorly motile spermatozoa and provide clues for finding molecular markers of these pathways.
登录
查看更多内容
通过比较蛋白质组学鉴定高活力和低活力水牛精子之间的差异
影响因子：
1.7
作者：
Huang, Y-L;Fu, Q.;Zhang, M.
通讯作者：
Zhang, M.
影响因子：
2.5
作者：
Hüttemann, M;Jaradat, S;Grossman, LI
通讯作者：
Grossman, LI
影响因子：
3
作者：
Chen J;Xu H;Aronow BJ;Jegga AG
通讯作者：
Jegga AG
影响因子：
3.9
作者：
Antony, Dinu;Becker-Heck, Anita;Zariwala, Maimoona A.;Schmidts, Miriam;Onoufriadis, Alexandros;Forouhan, Mitra;Wilson, Robert;Taylor-Cox, Theresa;Dewar, Ann;Jackson, Claire;Goggin, Patricia;Loges, Niki T.;Olbrich, Heike;Jaspers, Martine;Jorissen, Mark;Leigh, Margaret W.;Wolf, Whitney E.;Daniels, M. Leigh Anne;Noone, Peadar G.;Ferkol, Thomas W.;Sagel, Scott D.;Rosenfeld, Margaret;Rutman, Andrew;Dixit, Abhijit;O'Callaghan, Christopher;Lucas, Jane S.;Hogg, Claire;Scambler, Peter J.;Emes, Richard D.;Chung, Eddie M. K.;Shoemark, Amelia;Knowles, Michael R.;Omran, Heymut;Mitchison, Hannah M.
通讯作者：
Mitchison, Hannah M.
影响因子：
--
作者：
Cao, Wenlei;Ijiri, Takashi W.;Gerton, George L.
通讯作者：
Gerton, George L.