Hsa_circ_0008309 May Be a Potential Biomarker for Oral Squamous Cell Carcinoma.
Hsa_circ_0008309 May Be a Potential Biomarker for Oral Squamous Cell Carcinoma.
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Hsa_circ_0008309 可能是口腔鳞状细胞癌的潜在生物标志物
DOI：
10.1155/2018/7496890
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Li B;Wang F;Li X;Sun S;Shen Y;Yang H
目的口腔鳞状细胞癌（Oral squamous cell carcinoma，OSCC）是头颈部最常见的恶性肿瘤。已知环状RNA（circRNA）在不同类型癌症的致癌作用中起重要作用。然而，CircRNA在OSCC中的作用仍不清楚。方法采用高通量测序法检测8对口腔鳞癌组织及癌旁正常组织中circRNA的表达，并选取45对口腔鳞癌组织，采用逆转录-定量聚合酶链反应（RT-qPCR）法验证差异显著的circRNA。为了进一步研究hsa_circ_0008309的作用，使用生物信息学数据库预测circRNA-microRNA（miR）-mRNA网络。RT-qPCR检测hsa_circ_0008309、miR-1290、miR-136- 5 P和miR-382- 5 P在SCC-15和CAL 27细胞系中的表达水平。Western blotting检测Ataxin 1（ATXN 1）蛋白表达水平。结果高通量测序结果显示，circRNA在口腔鳞癌组织中大量表达，其中16条circRNA差异表达。Hsa_circ_0008309在45对口腔鳞癌组织标本中表达显著下调，且与病理分化程度相关。生物信息学数据库提示hsa_circ_0008309可分别与miR-1290、miR-136- 5 P和miR-382- 5 P联合收割机结合，调控ATXN 1的表达。随后确定hsa_circ_0008309可以抑制miR-136- 5 P和miR-382- 5 P表达并增加OSCC细胞系中ATXN 1的表达。结论口腔鳞癌组织中存在丰富的circRNA，首次探讨了hsa_circ_0008309-miR-136- 5 P/hsa-miR-382- 5 P-ATXN 1网络在口腔鳞癌中的调控作用。结果表明hsa_circ_0008309可能是口腔鳞癌的潜在生物标志物。
Objective Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and neck region. The circular RNA (circRNA) is known to serve an important role in the carcinogenesis of different types of cancer. However, the circRNA role of OSCC remains unclear. Methods 8 pairs of OSCC tissues and adjacent normal tissues were obtained to detect circRNAs expression by high-throughput sequencing, and 45 pairs of OSCC tissues were selected to verify the differentially significant circRNAs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To further investigate the role of hsa_circ_0008309, the circRNA-microRNA (miR)-mRNA network was predicted using bioinformatics databases. The expression levels of hsa_circ_0008309, miR-1290, miR-136-5P, and miR-382-5P in SCC-15 and CAL27 cell lines were detected by RT-qPCR. Western blotting was performed to detect the protein level of Ataxin 1 (ATXN1). Results The high-throughput sequencing results demonstrated that circRNAs were abundantly expressed in OSCC, and 16 circRNAs were significantly differentially expressed. Hsa_circ_0008309 was significantly downregulated in 45 pairs of OSCC tissue samples and was statistically correlated with pathological differentiation. The bioinformatics databases suggested that hsa_circ_0008309 could combine with miR-1290, miR-136-5P, and miR-382-5P, respectively, to regulate the expression of ATXN1. It was subsequently identified that hsa_circ_0008309 may inhibit miR-136-5P and miR-382-5P expression and increase ATXN1 expression in the OSCC cell lines. Conclusion In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and, to the best of our knowledge, we firstly explore the regulatory role of the hsa_circ_0008309-miR-136-5P/hsa-miR-382-5P-ATXN1 network in OSCC. The results indicated that hsa_circ_0008309 may be a potential biomarker for OSCC.
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