Method for analysis of nanoparticle hemolytic properties in vitro.
Method for analysis of nanoparticle hemolytic properties in vitro.
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DOI：
10.1021/nl0805615
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发表时间：
2008-08
期刊：
影响因子：
10.8
通讯作者：
McNeil, Scott E.
中科院分区：
文献类型：
--
作者：
Dobrovoiskaia, Marina A.;Clogston, Jeffrey D.;Neun, Barry W.;Hall, Jennifer B.;Patri, Anil K.;McNeil, Scott E.
体内溶血（红细胞的破坏）可导致贫血、黄疸和其他病理状况，因此必须对所有静脉给药的药物的溶血潜力进行评估。纳米技术衍生的器械和药物载体正作为传统小分子药物的替代品出现，对其与血液成分的生物相容性进行体外评估是临床前早期开发的必要部分。纳米颗粒的小尺寸和独特的物理化学性质可能导致它们与红细胞的相互作用不同于传统药物所观察到的情况，还可能干扰标准化的体外试验。将真正的溶血反应与颗粒干扰导致的假阳性或假阴性结果区分开来，对于正确解读这些试验非常重要。在此我们描述了一种用于分析纳米颗粒溶血特性的体外试验的验证，并讨论了观察到的纳米颗粒对该试验的干扰。我们提出了避免纳米颗粒产生误导性结果的替代方法，并讨论了纳米颗粒体外溶血特性与体内系统的潜在相关性。
Hemolysis (destruction of red blood cells) in vivo can lead to anemia, jaundice and other pathological conditions, therefore the hemolytic potential of all intravenously administered pharmaceuticals must be evaluated. Nanotechnology-derived devices and drug carriers are emerging as alternatives to conventional small-molecule drugs, and in vitro evaluation of their biocompatibility with blood components is a necessary part of early preclinical development. The small size and unique physicochemical properties of nanoparticles may cause their interactions with erythrocytes to differ from those observed for conventional pharmaceuticals, and may also cause interference with standardized in vitro tests. Separating true hemolytic responses from the false-positive or false-negative results caused by particle interference is important for correct interpretation of these tests. Here we describe validation of an in vitro assay for the analysis of nanoparticle hemolytic properties, and discuss observed nano-interferences with the assay. We propose alternative methods to avoid misleading results from nanoparticles, and discuss the potential relevance of nanoparticle in vitro hemolytic properties to in vivo systems.
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