Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony (Paeonia suffruticosa Andr.) flowers.
Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony (Paeonia suffruticosa Andr.) flowers.
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结合全长转录组学和代谢组学，揭示了牡丹黄色素形成的调控机制。鲜花。
DOI：
10.1038/s41438-021-00666-0
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发表时间：
2021-11-01
影响因子：
8.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Luo X;Sun D;Wang S;Luo S;Fu Y;Niu L;Shi Q;Zhang Y
Tree peony (Paeonia suffruticosa Andr.) is a popular ornamental plant in China due to its showy and colorful flowers. However, yellow-colored flowers are rare in both wild species and domesticated cultivars. The molecular mechanisms underlying yellow pigmentation remain poorly understood. Here, petal tissues of two tree peony cultivars, “High Noon” (yellow flowers) and “Roufurong” (purple–red flowers), were sampled at five developmental stages (S1–S5) from early flower buds to full blooms. Five petal color indices (brightness, redness, yellowness, chroma, and hue angle) and the contents of ten different flavonoids were determined. Compared to “Roufurong,” which accumulated abundant anthocyanins at S3–S5, the yellow-colored “High Noon” displayed relatively higher contents of tetrahydroxychalcone (THC), flavones, and flavonols but no anthocyanin production. The contents of THC, flavones, and flavonols in “High Noon” peaked at S3 and dropped gradually as the flower bloomed, consistent with the color index patterns. Furthermore, RNA-seq analyses at S3 showed that structural genes such as PsC4Hs, PsDFRs, and PsUFGTs in the flavonoid biosynthesis pathway were downregulated in “High Noon,” whereas most PsFLSs, PsF3Hs, and PsF3’Hs were upregulated. Five transcription factor (TF) genes related to flavonoid biosynthesis were also upregulated in “High Noon.” One of these TFs, PsMYB111, was overexpressed in tobacco, which led to increased flavonols but decreased anthocyanins. Dual-luciferase assays further confirmed that PsMYB111 upregulated PsFLS. These results improve our understanding of yellow pigmentation in tree peony and provide a guide for future molecular-assisted breeding experiments in tree peony with novel flower colors.
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影响因子：
5.6
作者：
Fan, Jinling;Zhu, Wenxue;Tao, Guanjun
通讯作者：
Tao, Guanjun
影响因子：
5.6
作者：
Gao L;Yang H;Liu H;Yang J;Hu Y
通讯作者：
Hu Y
DOI：
10.3390/molecules23020246
发表时间：
2018-01-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Guo S;Guo L;Zhao W;Xu J;Li Y;Zhang X;Shen X;Wu M;Hou X
通讯作者：
Hou X
DOI：
10.2503/jjshs.60.395
发表时间：
1991-09-01
影响因子：
--
作者：
HOSOKI, T;HAMADA, M;INABA, K
通讯作者：
INABA, K
影响因子：
7
作者：
Huddleston J;Ranade S;Malig M;Antonacci F;Chaisson M;Hon L;Sudmant PH;Graves TA;Alkan C;Dennis MY;Wilson RK;Turner SW;Korlach J;Eichler EE
通讯作者：
Eichler EE