Very low carbohydrate diet significantly alters the serum metabolic profiles in obese subjects.
Very low carbohydrate diet significantly alters the serum metabolic profiles in obese subjects.
复制标题
DOI：
10.1021/pr4008199
复制
发表时间：
2013-12-06
影响因子：
4.4
通讯作者：
Jia W
中科院分区：
文献类型：
--
作者：
Gu Y;Zhao A;Huang F;Zhang Y;Liu J;Wang C;Jia W;Xie G;Jia W
不断出现的证据表明，极低碳水化合物饮食（VLCD）可以防止肥胖的发展，但其潜在机制尚未完全了解。在这里，我们应用了一个全面的代谢组学方法，使用超高效液相色谱-四极杆飞行时间质谱和气相色谱-飞行时间质谱研究8周的饮食干预与VLCD对肥胖受试者的血清代谢谱的影响。VLCD干预导致体重减轻和稳态模型评估-胰岛素抵抗显著降低。代谢组学分析鉴定了与健康对照相比，肥胖受试者中主要归因于脂肪酸、氨基酸（包括支链氨基酸）、胺、脂质、羧酸和碳水化合物的许多差异血清代谢物（p < 0.05）。时间、VLCD干预与临床参数的相关性分析显示，代谢物的变化与临床参数的变化相关，且在男性和女性中存在差异。肥胖者的脂肪酸、氨基酸和羧酸含量高于正常健康者。VLCD摄入后，血清代谢物水平的这种增加被减弱，表明VLCD的健康有益作用与受损的脂肪酸和氨基酸代谢的减弱有关。VLCD诱导的显著代谢改变与肥胖相关的代谢变化无关。VLCD干预后肥胖受试者中改变的代谢物包括花生四烯酸、顺式-11，14-二十碳二烯酸、顺式-11，14，17-二十碳三烯酸、2-氨基丁酸、乙酰肉毒碱和苏糖酸，所有这些都参与炎症和氧化过程。结果显示，肥胖受试者摄入VLCD后脂肪酸和氨基酸发生了有利的变化，这应该被视为评估VLCD和类似饮食干预对健康有益影响的生物标志物。
Emerging evidence has consistently shown that very low carbohydrate diet (VLCD) can protect against the development of obesity, but the underlying mechanisms are not fully understood. Here we applied a comprehensive metabonomics approach using an ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry and gas chromatography-time of flight mass spectrometry to study the effects of an 8-week dietary intervention with VLCD on serum metabolic profiles in obese subjects. The VLCD intervention resulted in a weight loss and significantly decreased homeostasis model assessment-insulin resistance. The metabonomics analysis identified a number of differential serum metabolites (p < 0.05) primarily attributable to fatty acids, amino acids including branched chain amino acids, amines, lipids, carboxylic acids, and carbohydrates in obese subjects compared to healthy controls. The correlation analysis among time, VLCD intervention and clinical parameters revealed that the changes of metabolites correlated with the changes of clinical parameters and showed difference in males and females. Fatty acids, amino acids and carboxylic acids were increased in obese subjects compared with their normal healthy counterparts. Such increased levels of serum metabolites were attenuated after VLCD intake, suggesting that the health beneficial effects of VLCD are associated with attenuation of impaired fatty acid and amino acid metabolism. It also appears that VLCD induced significant metabolic alterations independent of the obesity-related metabolic changes. The altered metabolites in obese subjects post-VLCD intervention include arachidonate, cis-11,14-eicosadienoate, cis-11,14,17-eicosatrienoate, 2-aminobutyrate, acetyl-carnitine, and threonate, all of which are involved in inflammation and oxidation processes. The results revealed favorable shifts in fatty acids and amino acids after VLCD intake in obese subjects, which should be considered biomarkers for evaluating health beneficial effects of VLCD and similar dietary interventions.
登录
查看更多内容
影响因子：
9.8
作者：
Kalogeropoulo, Dionysia;LaFave, Laura;Nuttall, Frank Q.
通讯作者：
Nuttall, Frank Q.
影响因子：
39.2
作者：
Boden, G;Sargrad, K;Stein, TP
通讯作者：
Stein, TP
影响因子：
8.2
作者：
McAuley, KA;Hopkins, CM;Mann, JI
通讯作者：
Mann, JI
影响因子：
8.2
作者：
Lim, E. L.;Hollingsworth, K. G.;Aribisala, B. S.;Chen, M. J.;Mathers, J. C.;Taylor, R.
通讯作者：
Taylor, R.
影响因子：
7.1
作者：
Gannon, MC;Nuttall, FQ;Hoover, H
通讯作者：
Hoover, H